Difference between revisions of "List of plasmids"

From SubtiWiki
Jump to: navigation, search
(Labelling of a protein with a triple FLAG tag)
Line 8: Line 8:
  
 
==Labelling of a protein with a triple FLAG tag==
 
==Labelling of a protein with a triple FLAG tag==
* [[pGP1331]]: integrative plasmid for the fusion of 3x FLAG tag to the C-terminus of a protein, keeping expression under the control of the natural promotor
+
* [[pGP1331]]: integrative plasmid for the fusion of 3x FLAG tag to the C-terminus of a protein, keeping expression under the control of the natural promoter
 +
* [[pGP1087]]: integrative plasmid for the fusion of 3x FLAG tag to the C-terminus of a protein, keeping expression under the control of the natural promoter, allowing the expression of downstream genes under the control of the IPTG-inducible Pspac promoter
  
 
==Expression/ purification in ''B. subtilis''==
 
==Expression/ purification in ''B. subtilis''==

Revision as of 15:20, 16 March 2010

Here you can find a list of Bacillus subtilis plasmids:

Fusion to reporter genes

  • pAC5: translational lacZ-fusions (chloramphenicol resistence)
  • pAC6: transcriptional lacZ-fusions (cat resistence)
  • pAC7: translational lacZ-fusions (kanamycin resistence)
  • pBaSysBioII: gfp transcriptional fusions for high-throughput analysis of gene expression PubMed

Labelling of a protein with a triple FLAG tag

  • pGP1331: integrative plasmid for the fusion of 3x FLAG tag to the C-terminus of a protein, keeping expression under the control of the natural promoter
  • pGP1087: integrative plasmid for the fusion of 3x FLAG tag to the C-terminus of a protein, keeping expression under the control of the natural promoter, allowing the expression of downstream genes under the control of the IPTG-inducible Pspac promoter

Expression/ purification in B. subtilis

  • pBQ200: overexpression of proteins in B. subtilis under control of a strong degQ36 promoter
  • pGP380: expression of proteins in B. subtilis allows fusion to a Strep-tag at the N-terminus of the protein
  • pGP382: expression of proteins in B. subtilis allows fusion to a Strep-tag at the C-terminus of the protein

Expression/ purification in E. coli

  • pWH844: expression of proteins in E. coli, allows fusion to a N-terminal His-tag
  • pGP570: expression of proteins in E. coli, allows fusion to a N-terminal His-tag that can be cleaved off
  • pGP172: expression of proteins in E. coli allows fusion to a Strep-tag at the N-terminus of the protein
  • pGP574: expression of proteins in E. coli allows fusion to a Strep-tag at the C-terminus of the protein