GuaC

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  • Description: GMP reductase

Gene name guaC
Synonyms yumD
Essential no
Product GMP reductase
Function purine salvage and interconversion
Metabolic function and regulation of this protein in SubtiPathways:
Purine synthesis, Nucleotides (regulation)
MW, pI 35 kDa, 6.078
Gene length, protein length 978 bp, 326 aa
Immediate neighbours yuzG, paiB
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
GuaC context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU32130

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Inosine 5'-phosphate + NH3 + NADP+ = guanosine 5'-phosphate + NADPH (according to Swiss-Prot)
  • Protein family: GuaC type 2 subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Operon:
  • Regulation:
    • repressed in the presence of purine nucleotides (PurR) PubMed
    • activated during growth in the presence of branched chain amino acids (CodY) PubMed
    • expression activated by glucose (3.3 fold) PubMed
    • repressed in the presence of adenine or adenosine(PurR) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Hans-Matti Blencke, Georg Homuth, Holger Ludwig, Ulrike Mäder, Michael Hecker, Jörg Stülke
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metab. Eng.: 2003, 5(2);133-49
[PubMed:12850135] [WorldCat.org] (P p)

Virginie Molle, Yoshiko Nakaura, Robert P Shivers, Hirotake Yamaguchi, Richard Losick, Yasutaro Fujita, Abraham L Sonenshein
Additional targets of the Bacillus subtilis global regulator CodY identified by chromatin immunoprecipitation and genome-wide transcript analysis.
J. Bacteriol.: 2003, 185(6);1911-22
[PubMed:12618455] [WorldCat.org] [DOI] (P p)

H H Saxild, K Brunstedt, K I Nielsen, H Jarmer, P Nygaard
Definition of the Bacillus subtilis PurR operator using genetic and bioinformatic tools and expansion of the PurR regulon with glyA, guaC, pbuG, xpt-pbuX, yqhZ-folD, and pbuO.
J. Bacteriol.: 2001, 183(21);6175-83
[PubMed:11591660] [WorldCat.org] [DOI] (P p)