Difference between revisions of "GcaD"

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=The gene=
 
=The gene=

Revision as of 08:44, 23 June 2009

  • Description: UDP-N-acetylglucosamine pyrophosphorylase

Gene name gcaD
Synonyms tms, tms26
Essential yes PubMed
Product UDP-N-acetylglucosamine pyrophosphorylase
Function cell wall metabolism
Metabolic function and regulation of this protein in SubtiPathways:
Nucleotides (regulation)
MW, pI 49 kDa, 5.65
Gene length, protein length 1368 bp, 456 aa
Immediate neighbours spoVG, prs
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
GcaD context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU00500

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + N-acetyl-alpha-D-glucosamine 1-phosphate (according to Swiss-Prot)
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

I Hilden, B N Krath, B Hove-Jensen
Tricistronic operon expression of the genes gcaD (tms), which encodes N-acetylglucosamine 1-phosphate uridyltransferase, prs, which encodes phosphoribosyl diphosphate synthetase, and ctc in vegetative cells of Bacillus subtilis.
J. Bacteriol.: 1995, 177(24);7280-4
[PubMed:8522540] [WorldCat.org] [DOI] (P p)

B Hove-Jensen
Identification of tms-26 as an allele of the gcaD gene, which encodes N-acetylglucosamine 1-phosphate uridyltransferase in Bacillus subtilis.
J. Bacteriol.: 1992, 174(21);6852-6
[PubMed:1328164] [WorldCat.org] [DOI] (P p)