GatC

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  • Description: production of glutamyl-tRNA(Gln)

Gene name gatC
Synonyms yerL, yedA
Essential yes PubMed
Product glutamyl-tRNA(Gln) amidotransferase (subunit C)
Function translation
Metabolic function and regulation of this protein in SubtiPathways:
tRNA charging
MW, pI 10 kDa, 4.596
Gene length, protein length 288 bp, 96 aa
Immediate neighbours opuE, gatA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
GatC context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU06670

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate (according to Swiss-Prot)
  • Protein family: gatC family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Takuhiro Ito, Shigeyuki Yokoyama
Two enzymes bound to one transfer RNA assume alternative conformations for consecutive reactions.
Nature: 2010, 467(7315);612-6
[PubMed:20882017] [WorldCat.org] [DOI] (I p)

A W Curnow, K w Hong, R Yuan, S i Kim, O Martins, W Winkler, T M Henkin, D Söll
Glu-tRNAGln amidotransferase: a novel heterotrimeric enzyme required for correct decoding of glutamine codons during translation.
Proc. Natl. Acad. Sci. U.S.A.: 1997, 94(22);11819-26
[PubMed:9342321] [WorldCat.org] [DOI] (P p)