Difference between revisions of "GatC"

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= [[Categories]] containing this gene/protein =
 
= [[Categories]] containing this gene/protein =
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=== Database entries ===
 
=== Database entries ===
  
* '''Structure:'''
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* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?pdbId=2DF4 2DF4] ([[GatA]]-[[GatB]]-[[GatC]] complex from ''Staphylococcus aureus'') {{PubMed|16809541}}
  
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/O06492 O06492]
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/O06492 O06492]
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=gatC_728732_729022_1 gatC] {{PubMed|22383849}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=gatC_728732_729022_1 gatC] {{PubMed|22383849}}
  
* '''Sigma factor:'''  
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* '''[[Sigma factor]]:'''  
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
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=References=
 
=References=
  
<pubmed>9342321,20882017, 22517742</pubmed>
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<pubmed>9342321,20882017, 22517742 16809541</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 15:46, 12 July 2013

  • Description: production of glutamyl-tRNA(Gln)

Gene name gatC
Synonyms yerL, yedA
Essential yes PubMed
Product glutamyl-tRNA(Gln) amidotransferase (subunit C)
Function translation
Gene expression levels in SubtiExpress: gatC
Interactions involving this protein in SubtInteract: GatC
Metabolic function and regulation of this protein in SubtiPathways:
tRNA charging
MW, pI 10 kDa, 4.596
Gene length, protein length 288 bp, 96 aa
Immediate neighbours opuE, gatA
Sequences Protein DNA DNA_with_flanks
Genetic context
GatC context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
GatC expression.png















Categories containing this gene/protein

translation, essential genes, phosphoproteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU06670

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate (according to Swiss-Prot)
  • Protein family: gatC family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
    • phosphorylated on Arg-3 PubMed
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Alexander K W Elsholz, Kürsad Turgay, Stephan Michalik, Bernd Hessling, Katrin Gronau, Dan Oertel, Ulrike Mäder, Jörg Bernhardt, Dörte Becher, Michael Hecker, Ulf Gerth
Global impact of protein arginine phosphorylation on the physiology of Bacillus subtilis.
Proc. Natl. Acad. Sci. U.S.A.: 2012, 109(19);7451-6
[PubMed:22517742] [WorldCat.org] [DOI] (I p)

Takuhiro Ito, Shigeyuki Yokoyama
Two enzymes bound to one transfer RNA assume alternative conformations for consecutive reactions.
Nature: 2010, 467(7315);612-6
[PubMed:20882017] [WorldCat.org] [DOI] (I p)

Akiyoshi Nakamura, Min Yao, Sarin Chimnaronk, Naoki Sakai, Isao Tanaka
Ammonia channel couples glutaminase with transamidase reactions in GatCAB.
Science: 2006, 312(5782);1954-8
[PubMed:16809541] [WorldCat.org] [DOI] (I p)

A W Curnow, K w Hong, R Yuan, S i Kim, O Martins, W Winkler, T M Henkin, D Söll
Glu-tRNAGln amidotransferase: a novel heterotrimeric enzyme required for correct decoding of glutamine codons during translation.
Proc. Natl. Acad. Sci. U.S.A.: 1997, 94(22);11819-26
[PubMed:9342321] [WorldCat.org] [DOI] (P p)