Difference between revisions of "GatC"

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* '''Description:''' production of glutamyl-tRNA(Gln) <br/><br/>
 
 
 
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Revision as of 14:12, 10 August 2012

  • Description: production of glutamyl-tRNA(Gln)

Gene name gatC
Synonyms yerL, yedA
Essential yes PubMed
Product glutamyl-tRNA(Gln) amidotransferase (subunit C)
Function translation
Gene expression levels in SubtiExpress: gatC
Interactions involving this protein in SubtInteract: GatC
Metabolic function and regulation of this protein in SubtiPathways:
tRNA charging
MW, pI 10 kDa, 4.596
Gene length, protein length 288 bp, 96 aa
Immediate neighbours opuE, gatA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
GatC context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
GatC expression.png




























Categories containing this gene/protein

translation, essential genes, phosphoproteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU06670

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate (according to Swiss-Prot)
  • Protein family: gatC family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
    • phosphorylated on Arg-3 PubMed
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Alexander K W Elsholz, Kürsad Turgay, Stephan Michalik, Bernd Hessling, Katrin Gronau, Dan Oertel, Ulrike Mäder, Jörg Bernhardt, Dörte Becher, Michael Hecker, Ulf Gerth
Global impact of protein arginine phosphorylation on the physiology of Bacillus subtilis.
Proc. Natl. Acad. Sci. U.S.A.: 2012, 109(19);7451-6
[PubMed:22517742] [WorldCat.org] [DOI] (I p)

Takuhiro Ito, Shigeyuki Yokoyama
Two enzymes bound to one transfer RNA assume alternative conformations for consecutive reactions.
Nature: 2010, 467(7315);612-6
[PubMed:20882017] [WorldCat.org] [DOI] (I p)

A W Curnow, K w Hong, R Yuan, S i Kim, O Martins, W Winkler, T M Henkin, D Söll
Glu-tRNAGln amidotransferase: a novel heterotrimeric enzyme required for correct decoding of glutamine codons during translation.
Proc. Natl. Acad. Sci. U.S.A.: 1997, 94(22);11819-26
[PubMed:9342321] [WorldCat.org] [DOI] (P p)