Difference between revisions of "GamA"

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= [[Categories]] containing this gene/protein =
 
= [[Categories]] containing this gene/protein =
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= This gene is a member of the following [[regulons]] =
 
= This gene is a member of the following [[regulons]] =
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{{SubtiWiki regulon|[[YbgR regulon]]}}
  
  
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===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
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* no growth on glucosamine {{PubMed|23667565}}
  
 
=== Database entries ===
 
=== Database entries ===
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=References=
 
=References=
  
<pubmed>10627040, 14343123 </pubmed>
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<pubmed>10627040, 14343123 23667565</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 16:25, 24 May 2013

  • Description: glucosamine-6-phosphate deaminase

Gene name gamA
Synonyms ybfT
Essential no
Product glucosamine-6-phosphate deaminase
Function glucosamine utilization
Gene expression levels in SubtiExpress: gamA
Metabolic function and regulation of this protein in SubtiPathways:
Sugar catabolism
MW, pI 27 kDa, 5.793
Gene length, protein length 747 bp, 249 aa
Immediate neighbours gamP, ybgA
Sequences Protein DNA DNA_with_flanks
Genetic context
GamA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
GamA expression.png















Categories containing this gene/protein

cell wall degradation/ turnover, utilization of specific carbon sources

This gene is a member of the following regulons

YbgR regulon


The gene

Basic information

  • Locus tag: BSU02360

Phenotypes of a mutant

  • no growth on glucosamine PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: d-glucosamine 6-phosphate + H2O = D-fructose 6-phosphate + NH3 (according to Swiss-Prot)
  • Protein family: NagB subfamily (according to Swiss-Prot)
  • Paralogous protein(s): NagB

Extended information on the protein

  • Kinetic information: K(M): 3.0 mM PubMed
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
    • induced by N-acetylglucosamine PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Isabelle Gaugué, Jacques Oberto, Harald Putzer, Jacqueline Plumbridge
The use of amino sugars by Bacillus subtilis: presence of a unique operon for the catabolism of glucosamine.
PLoS One: 2013, 8(5);e63025
[PubMed:23667565] [WorldCat.org] [DOI] (I e)

Jonathan Reizer, Steffi Bachem, Aiala Reizer, Maryvonne Arnaud, Milton H Saier, Jörg Stülke
Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of Bacillus subtilis.
Microbiology (Reading): 1999, 145 ( Pt 12);3419-3429
[PubMed:10627040] [WorldCat.org] [DOI] (P p)

C J BATES, C A PASTERNAK
FURTHER STUDIES ON THE REGULATION OF AMINO SUGAR METABOLISM IN BACILLUS SUBTILIS.
Biochem J: 1965, 96;147-54
[PubMed:14343123] [WorldCat.org] [DOI] (P p)