From SubtiWiki
Revision as of 14:21, 17 April 2014 by (talk)
Jump to: navigation, search
  • Description: fructosamine kinase

Gene name frlD
Synonyms yurL
Essential no
Product fructosamine kinase
Function metabolism of sugar amines
Gene expression levels in SubtiExpress: frlD
Metabolic function and regulation of this protein in SubtiPathways:
MW, pI 30 kDa, 4.909
Gene length, protein length 852 bp, 284 aa
Immediate neighbours frlR, frlM
Sequences Protein DNA DNA_with_flanks
Genetic context
YurL context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
FrlD expression.png

Categories containing this gene/protein

utilization of specific carbon sources, utilization of nitrogen sources other than amino acids

This gene is a member of the following regulons

CodY regulon, FrlR regulon

The gene

Basic information

  • Locus tag: BSU32570

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: carbohydrate kinase pfkB family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
    • frlB: repressed during growth in the presence of branched chain amino acids (CodY) PubMed
    • frlB: induced in the presence of fructosamine (FrlR) PubMed
  • Additional information:
    • the mRNA is substantially stabilized upon depletion of RNase Y PubMed
    • the frlB-frlO-frlN-frlM-frlD operon is not expressed in a cshA mutant PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 828 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 9042 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 3514 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 4350 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks


Martin Lehnik-Habrink, Leonie Rempeters, Ákos T Kovács, Christoph Wrede, Claudia Baierlein, Heike Krebber, Oscar P Kuipers, Jörg Stülke
DEAD-Box RNA helicases in Bacillus subtilis have multiple functions and act independently from each other.
J Bacteriol: 2013, 195(3);534-44
[PubMed:23175651] [] [DOI] (I p)

Martin Lehnik-Habrink, Marc Schaffer, Ulrike Mäder, Christine Diethmaier, Christina Herzberg, Jörg Stülke
RNA processing in Bacillus subtilis: identification of targets of the essential RNase Y.
Mol Microbiol: 2011, 81(6);1459-73
[PubMed:21815947] [] [DOI] (I p)

Veronika Maria Deppe, Stephanie Klatte, Johannes Bongaerts, Karl-Heinz Maurer, Timothy O'Connell, Friedhelm Meinhardt
Genetic control of amadori product degradation in Bacillus subtilis via regulation of frlBONMD expression by FrlR.
Appl Environ Microbiol: 2011, 77(9);2839-46
[PubMed:21398478] [] [DOI] (I p)

Veronika Maria Deppe, Johannes Bongaerts, Timothy O'Connell, Karl-Heinz Maurer, Friedhelm Meinhardt
Enzymatic deglycation of Amadori products in bacteria: mechanisms, occurrence and physiological functions.
Appl Microbiol Biotechnol: 2011, 90(2);399-406
[PubMed:21347729] [] [DOI] (I p)

Elsa Wiame, Pedro Lamosa, Helena Santos, Emile Van Schaftingen
Identification of glucoselysine-6-phosphate deglycase, an enzyme involved in the metabolism of the fructation product glucoselysine.
Biochem J: 2005, 392(Pt 2);263-9
[PubMed:16153181] [] [DOI] (I p)

Elsa Wiame, Armelle Duquenne, Ghislain Delpierre, Emile Van Schaftingen
Identification of enzymes acting on alpha-glycated amino acids in Bacillus subtilis.
FEBS Lett: 2004, 577(3);469-72
[PubMed:15556630] [] [DOI] (P p)

Virginie Molle, Yoshiko Nakaura, Robert P Shivers, Hirotake Yamaguchi, Richard Losick, Yasutaro Fujita, Abraham L Sonenshein
Additional targets of the Bacillus subtilis global regulator CodY identified by chromatin immunoprecipitation and genome-wide transcript analysis.
J Bacteriol: 2003, 185(6);1911-22
[PubMed:12618455] [] [DOI] (P p)