Difference between revisions of "EpsA"

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(Biological materials)
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* '''Antibody:'''
 
* '''Antibody:'''
  
* '''FLAG-tag construct:''' GP1526 epsA-FLAG 3x spc available in [[ Jörg Stülke]]'s lab
+
* '''FLAG-tag construct:''' GP1526 epsA-FLAG 3x spc (based on [[pGP1331]]) available in [[ Jörg Stülke]]'s lab
  
 
=Labs working on this gene/protein=
 
=Labs working on this gene/protein=

Revision as of 11:34, 31 January 2014

  • Description: extracellular polysaccharide synthesis, putative transmembrane modulator of EpsB activity, might activate EpsB autophosphorylation and substrate phosphorylation

Gene name epsA
Synonyms yveK
Essential no
Product unknown
Function biofilm formation
Gene expression levels in SubtiExpress: epsA
Interactions involving this protein in SubtInteract: EpsA
Regulation of this protein in SubtiPathways:
epsA
MW, pI 25 kDa, 6.071
Gene length, protein length 702 bp, 234 aa
Immediate neighbours epsB, slrR
Sequences Protein DNA DNA_with_flanks
Genetic context
YveK context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
EpsA expression.png















Categories containing this gene/protein

protein modification, biofilm formation, membrane proteins

This gene is a member of the following regulons

AbrB regulon, RemA regulon, SinR regulon

The gene

Basic information

  • Locus tag: BSU34370

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: cpsC/capA family (according to Swiss-Prot)
  • Paralogous protein(s): TkmA

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion: GP1569 epsA-gfp (spc)
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
  • Antibody:

Labs working on this gene/protein

Richard Losick, Harvard Univ., Cambridge, USA homepage

Your additional remarks

References

Reviews

Massimiliano Marvasi, Pieter T Visscher, Lilliam Casillas Martinez
Exopolymeric substances (EPS) from Bacillus subtilis: polymers and genes encoding their synthesis.
FEMS Microbiol. Lett.: 2010, 313(1);1-9
[PubMed:20735481] [WorldCat.org] [DOI] (I p)


Original publications