Difference between revisions of "DacF"

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(Expression and regulation)
Line 94: Line 94:
* '''[[Sigma factor]]:''' [[SigF]] {{PubMed|15699190}}, [[SigG]]
* '''[[Sigma factor]]:''' [[SigF]] {{PubMed|15699190}}, [[SigG]]
* '''Regulation:''' repressed by casamino acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed] expressed during sporulation in the prespore [http://www.ncbi.nlm.nih.gov/sites/entrez/15758244 Link to a summary]
* '''Regulation:'''  
** repressed by casamino acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed]  
** expressed during sporulation in the forespore [http://www.ncbi.nlm.nih.gov/sites/entrez/15758244 PubMed]
* '''Regulatory mechanism:'''  
* '''Regulatory mechanism:'''  

Revision as of 22:11, 31 October 2009

  • Description: penicillin-binding protein I, D-alanyl-D-alanine carboxypeptidase

Gene name dacF
Essential no
Product penicillin-binding protein I,
D-alanyl-D-alanine carboxypeptidase
Function carboxypeptidase
Metabolic function and regulation of this protein in SubtiPathways:
MW, pI 43 kDa, 9.912
Gene length, protein length 1167 bp, 389 aa
Immediate neighbours spoIIAA, pupG
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
DacF context.gif
This image was kindly provided by SubtiList

The gene

Basic information

  • Locus tag: BSU23480

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala (according to Swiss-Prot)
  • Protein family: peptidase S11 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • repressed by casamino acids PubMed
    • expressed during sporulation in the forespore PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks


Stephanie T Wang, Barbara Setlow, Erin M Conlon, Jessica L Lyon, Daisuke Imamura, Tsutomu Sato, Peter Setlow, Richard Losick, Patrick Eichenberger
The forespore line of gene expression in Bacillus subtilis.
J Mol Biol: 2006, 358(1);16-37
[PubMed:16497325] [WorldCat.org] [DOI] (P p)

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)

D L Popham, M E Gilmore, P Setlow
Roles of low-molecular-weight penicillin-binding proteins in Bacillus subtilis spore peptidoglycan synthesis and spore properties.
J Bacteriol: 1999, 181(1);126-32
[PubMed:9864321] [WorldCat.org] [DOI] (P p)

Peter J Lewis, Jeffery Errington
Use of green fluorescent protein for detection of cell-specific gene expression and subcellular protein localization during sporulation in Bacillus subtilis.
Microbiology (Reading): 1996, 142 ( Pt 4);733-740
[PubMed:8936302] [WorldCat.org] [DOI] (P p)