Difference between revisions of "DacF"

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* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P38422 P38422]
 
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P38422 P38422]
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU23480]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU23480]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''

Revision as of 01:51, 25 June 2009

  • Description: penicillin-binding protein I, D-alanyl-D-alanine carboxypeptidase

Gene name dacF
Synonyms
Essential no
Product penicillin-binding protein I,
D-alanyl-D-alanine carboxypeptidase
Function carboxypeptidase
Metabolic function and regulation of this protein in SubtiPathways:
Stress
MW, pI 43 kDa, 9.912
Gene length, protein length 1167 bp, 389 aa
Immediate neighbours spoIIAA, punA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
DacF context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU23480

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala (according to Swiss-Prot)
  • Protein family: peptidase S11 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Stephanie T Wang, Barbara Setlow, Erin M Conlon, Jessica L Lyon, Daisuke Imamura, Tsutomu Sato, Peter Setlow, Richard Losick, Patrick Eichenberger
The forespore line of gene expression in Bacillus subtilis.
J Mol Biol: 2006, 358(1);16-37
[PubMed:16497325] [WorldCat.org] [DOI] (P p)

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)

D L Popham, M E Gilmore, P Setlow
Roles of low-molecular-weight penicillin-binding proteins in Bacillus subtilis spore peptidoglycan synthesis and spore properties.
J Bacteriol: 1999, 181(1);126-32
[PubMed:9864321] [WorldCat.org] [DOI] (P p)

P J Lewis, J Errington
Use of green fluorescent protein for detection of cell-specific gene expression and subcellular protein localization during sporulation in Bacillus subtilis.
Microbiology (Reading): 1996, 142 ( Pt 4);733-40
[PubMed:8936302] [WorldCat.org] [DOI] (P p)