Difference between revisions of "Ctc"

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=The gene=
 
=The gene=

Revision as of 08:27, 23 June 2009

  • Description: general stress protein, similar to L25

Gene name ctc
Synonyms
Essential no
Product ribosomal protein
Function unknown
Metabolic function and regulation of this protein in SubtiPathways:
Nucleotides (regulation)
MW, pI 21 kDa, 4.216
Gene length, protein length 612 bp, 204 aa
Immediate neighbours prs, spoVC
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
Ctc context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU00520

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: Ctc subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions: binds 5S rRNA PubMed
  • Localization: in the ribosome (large subunit) PubMed

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and regulation

  • Regulation: induced by stress (SigB) PubMed, induced under stress conditions
  • Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody: available in Stülke lab

Labs working on this gene/protein

Your additional remarks

References

G M Gongadze, A P Korepanov, A V Korobeinikova, M B Garber
Bacterial 5S rRNA-binding proteins of the CTC family.
Biochemistry Mosc.: 2008, 73(13);1405-17
[PubMed:19216708] [WorldCat.org] (I p)

Dirk Höper, Uwe Völker, Michael Hecker
Comprehensive characterization of the contribution of individual SigB-dependent general stress genes to stress resistance of Bacillus subtilis.
J. Bacteriol.: 2005, 187(8);2810-26
[PubMed:15805528] [WorldCat.org] [DOI] (P p)

Matthias Schmalisch, Ines Langbein, Jörg Stülke
The general stress protein Ctc of Bacillus subtilis is a ribosomal protein.
J. Mol. Microbiol. Biotechnol.: 2002, 4(5);495-501
[PubMed:12432960] [WorldCat.org] (P p)

I Hilden, B N Krath, B Hove-Jensen
Tricistronic operon expression of the genes gcaD (tms), which encodes N-acetylglucosamine 1-phosphate uridyltransferase, prs, which encodes phosphoribosyl diphosphate synthetase, and ctc in vegetative cells of Bacillus subtilis.
J. Bacteriol.: 1995, 177(24);7280-4
[PubMed:8522540] [WorldCat.org] [DOI] (P p)

C L Truitt, E A Weaver, W G Haldenwang
Effects on growth and sporulation of inactivation of a Bacillus subtilis gene (ctc) transcribed in vitro by minor vegetative cell RNA polymerases (E-sigma 37, E-sigma 32).
Mol. Gen. Genet.: 1988, 212(1);166-71
[PubMed:2836704] [WorldCat.org] [DOI] (P p)

C Ray, M Igo, W Shafer, R Losick, C P Moran
Suppression of ctc promoter mutations in Bacillus subtilis.
J. Bacteriol.: 1988, 170(2);900-7
[PubMed:3123466] [WorldCat.org] [DOI] (P p)

M M Igo, R Losick
Regulation of a promoter that is utilized by minor forms of RNA polymerase holoenzyme in Bacillus subtilis.
J. Mol. Biol.: 1986, 191(4);615-24
[PubMed:3100810] [WorldCat.org] [DOI] (P p)

J F Ollington, W G Haldenwang, T V Huynh, R Losick
Developmentally regulated transcription in a cloned segment of the Bacillus subtilis chromosome.
J. Bacteriol.: 1981, 147(2);432-42
[PubMed:6790515] [WorldCat.org] (P p)

W G Haldenwang, R Losick
A modified RNA polymerase transcribes a cloned gene under sporulation control in Bacillus subtilis.
Nature: 1979, 282(5736);256-60
[PubMed:116131] [WorldCat.org] [DOI] (P p)