Difference between revisions of "ComK"

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=The gene=
=The gene=

Revision as of 08:26, 23 June 2009

  • Description: competence transcription factor (CTF)

Gene name comK
Essential no
Product competence transcription factor (CTF)
Function regulation of competence and DNA uptake
Metabolic function and regulation of this protein in SubtiPathways:
Nucleotides (regulation)
MW, pI 22 kDa, 7.669
Gene length, protein length 576 bp, 192 aa
Immediate neighbours yhzC, yhxD
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
ComK context.gif
This image was kindly provided by SubtiList

The gene

Basic information

  • Locus tag: BSU10420

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation: repressed by casamino acids PubMed
    • repressed by Rok PubMed
    • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
    • repressed during logrithmic growth (AbrB) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Oscar Kuipers, University of Groningen, The Netherlands Homepage

Your additional remarks


Wiep Klaas Smits, Cristina Bongiorni, Jan-Willem Veening, Leendert W Hamoen, Oscar P Kuipers, Marta Perego
Temporal separation of distinct differentiation pathways by a dual specificity Rap-Phr system in Bacillus subtilis.
Mol. Microbiol.: 2007, 65(1);103-20
[PubMed:17581123] [WorldCat.org] [DOI] (P p)

Hédia Maamar, Arjun Raj, David Dubnau
Noise in gene expression determines cell fate in Bacillus subtilis.
Science: 2007, 317(5837);526-9
[PubMed:17569828] [WorldCat.org] [DOI] (I p)

Peter Prepiak, David Dubnau
A peptide signal for adapter protein-mediated degradation by the AAA+ protease ClpCP.
Mol. Cell: 2007, 26(5);639-47
[PubMed:17560370] [WorldCat.org] [DOI] (P p)

Wiep Klaas Smits, Tran Thu Hoa, Leendert W Hamoen, Oscar P Kuipers, David Dubnau
Antirepression as a second mechanism of transcriptional activation by a minor groove binding protein.
Mol. Microbiol.: 2007, 64(2);368-81
[PubMed:17493123] [WorldCat.org] [DOI] (P p)

Kim A Susanna, Fabrizia Fusetti, Andy-Mark W H Thunnissen, Leendert W Hamoen, Oscar P Kuipers
Functional analysis of the competence transcription factor ComK of Bacillus subtilis by characterization of truncation variants.
Microbiology (Reading, Engl.): 2006, 152(Pt 2);473-83
[PubMed:16436435] [WorldCat.org] [DOI] (P p)

Kim A Susanna, Chris D den Hengst, Leendert W Hamoen, Oscar P Kuipers
Expression of transcription activator ComK of Bacillus subtilis in the heterologous host Lactococcus lactis leads to a genome-wide repression pattern: a case study of horizontal gene transfer.
Appl. Environ. Microbiol.: 2006, 72(1);404-11
[PubMed:16391071] [WorldCat.org] [DOI] (P p)

Hédia Maamar, David Dubnau
Bistability in the Bacillus subtilis K-state (competence) system requires a positive feedback loop.
Mol. Microbiol.: 2005, 56(3);615-24
[PubMed:15819619] [WorldCat.org] [DOI] (P p)

Leendert W Hamoen, Daisy Kausche, Mohamed A Marahiel, Douwe van Sinderen, Gerard Venema, Pascale Serror
The Bacillus subtilis transition state regulator AbrB binds to the -35 promoter region of comK.
FEMS Microbiol. Lett.: 2003, 218(2);299-304
[PubMed:12586407] [WorldCat.org] [DOI] (P p)

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J. Bacteriol.: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)

Michiko M Nakano, Shunji Nakano, Peter Zuber
Spx (YjbD), a negative effector of competence in Bacillus subtilis, enhances ClpC-MecA-ComK interaction.
Mol. Microbiol.: 2002, 44(5);1341-9
[PubMed:12028382] [WorldCat.org] [DOI] (P p)

Mitsuo Ogura, Hirotake Yamaguchi, Kazuo Kobayashi, Naotake Ogasawara, Yasutaro Fujita, Teruo Tanaka
Whole-genome analysis of genes regulated by the Bacillus subtilis competence transcription factor ComK.
J. Bacteriol.: 2002, 184(9);2344-51
[PubMed:11948146] [WorldCat.org] [DOI] (P p)

Randy M Berka, Jeanette Hahn, Mark Albano, Irena Draskovic, Marjan Persuh, Xianju Cui, Alan Sloma, William Widner, David Dubnau
Microarray analysis of the Bacillus subtilis K-state: genome-wide expression changes dependent on ComK.
Mol. Microbiol.: 2002, 43(5);1331-45
[PubMed:11918817] [WorldCat.org] [DOI] (P p)

Tran Thu Hoa, Pablo Tortosa, Mark Albano, David Dubnau
Rok (YkuW) regulates genetic competence in Bacillus subtilis by directly repressing comK.
Mol. Microbiol.: 2002, 43(1);15-26
[PubMed:11849533] [WorldCat.org] [DOI] (P p)

Hanne Jarmer, Randy Berka, Steen Knudsen, Hans H Saxild
Transcriptome analysis documents induced competence of Bacillus subtilis during nitrogen limiting conditions.
FEMS Microbiol. Lett.: 2002, 206(2);197-200
[PubMed:11814663] [WorldCat.org] [DOI] (P p)

M M Nakano, F Hajarizadeh, Y Zhu, P Zuber
Loss-of-function mutations in yjbD result in ClpX- and ClpP-independent competence development of Bacillus subtilis.
Mol. Microbiol.: 2001, 42(2);383-94
[PubMed:11703662] [WorldCat.org] [DOI] (P p)

L W Hamoen, A F Van Werkhoven, G Venema, D Dubnau
The pleiotropic response regulator DegU functions as a priming protein in competence development in Bacillus subtilis.
Proc. Natl. Acad. Sci. U.S.A.: 2000, 97(16);9246-51
[PubMed:10908654] [WorldCat.org] [DOI] (P p)

M Persuh, K Turgay, I Mandic-Mulec, D Dubnau
The N- and C-terminal domains of MecA recognize different partners in the competence molecular switch.
Mol. Microbiol.: 1999, 33(4);886-94
[PubMed:10447896] [WorldCat.org] [DOI] (P p)

M Ogura, L Liu, M Lacelle, M M Nakano, P Zuber
Mutational analysis of ComS: evidence for the interaction of ComS and MecA in the regulation of competence development in Bacillus subtilis.
Mol. Microbiol.: 1999, 32(4);799-812
[PubMed:10361283] [WorldCat.org] [DOI] (P p)

K Turgay, J Hahn, J Burghoorn, D Dubnau
Competence in Bacillus subtilis is controlled by regulated proteolysis of a transcription factor.
EMBO J.: 1998, 17(22);6730-8
[PubMed:9890793] [WorldCat.org] [DOI] (P p)

L W Hamoen, A F Van Werkhoven, J J Bijlsma, D Dubnau, G Venema
The competence transcription factor of Bacillus subtilis recognizes short A/T-rich sequences arranged in a unique, flexible pattern along the DNA helix.
Genes Dev.: 1998, 12(10);1539-50
[PubMed:9585513] [WorldCat.org] [DOI] (P p)

M Ogura, Y Ohshiro, S Hirao, T Tanaka
A new Bacillus subtilis gene, med, encodes a positive regulator of comK.
J. Bacteriol.: 1997, 179(20);6244-53
[PubMed:9335269] [WorldCat.org] [DOI] (P p)

M Ogura, T Tanaka
Bacillus subtilis ComK negatively regulates degR gene expression.
Mol. Gen. Genet.: 1997, 254(2);157-65
[PubMed:9108277] [WorldCat.org] [DOI] (P p)

K Turgay, L W Hamoen, G Venema, D Dubnau
Biochemical characterization of a molecular switch involving the heat shock protein ClpC, which controls the activity of ComK, the competence transcription factor of Bacillus subtilis.
Genes Dev.: 1997, 11(1);119-28
[PubMed:9000055] [WorldCat.org] [DOI] (P p)

P Serror, A L Sonenshein
CodY is required for nutritional repression of Bacillus subtilis genetic competence.
J. Bacteriol.: 1996, 178(20);5910-5
[PubMed:8830686] [WorldCat.org] [DOI] (P p)

J Hahn, A Luttinger, D Dubnau
Regulatory inputs for the synthesis of ComK, the competence transcription factor of Bacillus subtilis.
Mol. Microbiol.: 1996, 21(4);763-75
[PubMed:8878039] [WorldCat.org] [DOI] (P p)

D van Sinderen, A Luttinger, L Kong, D Dubnau, G Venema, L Hamoen
comK encodes the competence transcription factor, the key regulatory protein for competence development in Bacillus subtilis.
Mol. Microbiol.: 1995, 15(3);455-62
[PubMed:7783616] [WorldCat.org] [DOI] (P p)

B J Haijema, L W Hamoen, J Kooistra, G Venema, D van Sinderen
Expression of the ATP-dependent deoxyribonuclease of Bacillus subtilis is under competence-mediated control.
Mol. Microbiol.: 1995, 15(2);203-11
[PubMed:7746142] [WorldCat.org] [DOI] (P p)

L Kong, D Dubnau
Regulation of competence-specific gene expression by Mec-mediated protein-protein interaction in Bacillus subtilis.
Proc. Natl. Acad. Sci. U.S.A.: 1994, 91(13);5793-7
[PubMed:8016067] [WorldCat.org] [DOI] (P p)