Difference between revisions of "ComGA"

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(Biological materials)
(Biological materials)
Line 143: Line 143:
* '''GFP fusion:'''  
* '''GFP fusion:'''  
GP1640 ''trpC2'' ''[[ymdB]]''::spc ''comG-gfp'' (''spc''), available in [[ Jörg Stülke]]'s lab
**GP1640 ''trpC2'' ''[[ymdB]]''::spc ''comG-gfp'' (''spc''), available in [[ Jörg Stülke]]'s lab
GP1643 ''comG-gfp'' (''spc'') in the NCIB3610 background, available in [[ Jörg Stülke]]'s lab
**GP1643 ''comG-gfp'' (''spc'') in the NCIB3610 background, available in [[ Jörg Stülke]]'s lab
* '''two-hybrid system:'''  
* '''two-hybrid system:'''  

Revision as of 15:48, 3 February 2015

  • Description: late competence protein, traffic ATPase, binds and transports transforming DNA

Gene name comGA
Essential no
Product traffic ATPase
Function genetic competence
Gene expression levels in SubtiExpress: comGA
Interactions involving this protein in SubtInteract: ComGA
Metabolic function and regulation of this protein in SubtiPathways:
MW, pI 40 kDa, 8.893
Gene length, protein length 1068 bp, 356 aa
Immediate neighbours comGB, corA
Sequences Protein DNA DNA_with_flanks
Genetic context
ComGA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
ComGA expression.png

Categories containing this gene/protein

genetic competence, membrane proteins, phosphoproteins

This gene is a member of the following regulons

ComK regulon

The gene

Basic information

  • Locus tag: BSU24730

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: binds and transports transforming DNA
  • Protein family: GSP E family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
    • phosphorylated on Arg-28, Arg-71- and Arg-330 PubMed
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • repressed by casamino acids PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
    • GP1640 trpC2 ymdB::spc comG-gfp (spc), available in Jörg Stülke's lab
    • GP1643 comG-gfp (spc) in the NCIB3610 background, available in Jörg Stülke's lab
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks



Inês Chen, David Dubnau
DNA uptake during bacterial transformation.
Nat. Rev. Microbiol.: 2004, 2(3);241-9
[PubMed:15083159] [WorldCat.org] [DOI] (P p)

Original publications

Alexander K W Elsholz, Kürsad Turgay, Stephan Michalik, Bernd Hessling, Katrin Gronau, Dan Oertel, Ulrike Mäder, Jörg Bernhardt, Dörte Becher, Michael Hecker, Ulf Gerth
Global impact of protein arginine phosphorylation on the physiology of Bacillus subtilis.
Proc. Natl. Acad. Sci. U.S.A.: 2012, 109(19);7451-6
[PubMed:22517742] [WorldCat.org] [DOI] (I p)

Kenneth Briley, Angella Dorsey-Oresto, Peter Prepiak, Miguel J Dias, Jessica M Mann, David Dubnau
The secretion ATPase ComGA is required for the binding and transport of transforming DNA.
Mol. Microbiol.: 2011, 81(3);818-30
[PubMed:21707789] [WorldCat.org] [DOI] (I p)

Kenneth Briley, Peter Prepiak, Miguel J Dias, Jeanette Hahn, David Dubnau
Maf acts downstream of ComGA to arrest cell division in competent cells of B. subtilis.
Mol. Microbiol.: 2011, 81(1);23-39
[PubMed:21564336] [WorldCat.org] [DOI] (I p)

Miriam Kaufenstein, Martin van der Laan, Peter L Graumann
The three-layered DNA uptake machinery at the cell pole in competent Bacillus subtilis cells is a stable complex.
J. Bacteriol.: 2011, 193(7);1633-42
[PubMed:21278288] [WorldCat.org] [DOI] (I p)

Naomi Kramer, Jeanette Hahn, David Dubnau
Multiple interactions among the competence proteins of Bacillus subtilis.
Mol. Microbiol.: 2007, 65(2);454-64
[PubMed:17630974] [WorldCat.org] [DOI] (P p)

Jeanette Hahn, Berenike Maier, Bert Jan Haijema, Michael Sheetz, David Dubnau
Transformation proteins and DNA uptake localize to the cell poles in Bacillus subtilis.
Cell: 2005, 122(1);59-71
[PubMed:16009133] [WorldCat.org] [DOI] (P p)

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J. Bacteriol.: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)

Hanne Jarmer, Randy Berka, Steen Knudsen, Hans H Saxild
Transcriptome analysis documents induced competence of Bacillus subtilis during nitrogen limiting conditions.
FEMS Microbiol. Lett.: 2002, 206(2);197-200
[PubMed:11814663] [WorldCat.org] [DOI] (P p)

B J Haijema, J Hahn, J Haynes, D Dubnau
A ComGA-dependent checkpoint limits growth during the escape from competence.
Mol. Microbiol.: 2001, 40(1);52-64
[PubMed:11298275] [WorldCat.org] [DOI] (P p)

Y S Chung, F Breidt, D Dubnau
Cell surface localization and processing of the ComG proteins, required for DNA binding during transformation of Bacillus subtilis.
Mol. Microbiol.: 1998, 29(3);905-13
[PubMed:9723928] [WorldCat.org] [DOI] (P p)

Y S Chung, D Dubnau
All seven comG open reading frames are required for DNA binding during transformation of competent Bacillus subtilis.
J. Bacteriol.: 1998, 180(1);41-5
[PubMed:9422590] [WorldCat.org] (P p)

D van Sinderen, A ten Berge, B J Hayema, L Hamoen, G Venema
Molecular cloning and sequence of comK, a gene required for genetic competence in Bacillus subtilis.
Mol. Microbiol.: 1994, 11(4);695-703
[PubMed:8196543] [WorldCat.org] [DOI] (P p)

M Albano, R Breitling, D A Dubnau
Nucleotide sequence and genetic organization of the Bacillus subtilis comG operon.
J. Bacteriol.: 1989, 171(10);5386-404
[PubMed:2507524] [WorldCat.org] [DOI] (P p)