CitG

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  • Description: fumarase

Gene name citG
Synonyms
Essential no
Product fumarate hydratase
Function TCA cycle
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 50 kDa, 5.475
Gene length, protein length 1386 bp, 462 aa
Immediate neighbours yuxN, gerAA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
CitG context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU33040

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: (S)-malate = fumarate + H2O (according to Swiss-Prot)
  • Protein family: Fumarase subfamily (according to Swiss-Prot)
  • Paralogous protein(s): AnsB

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure: 1YFE (E.coli)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation: constitutive
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: GP718 (spec), available in Stülke lab
  • Expression vector:
    • pGP1122 (N-terminal Strep-tag, for SPINE, purification from B. subtilis, in pGP380) (available in Stülke lab)
  • lacZ fusion: pGP387 (in pAC7), available in Stülke lab
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

K M Tatti, H L Carter, A Moir, C P Moran
Sigma H-directed transcription of citG in Bacillus subtilis.
J. Bacteriol.: 1989, 171(11);5928-32
[PubMed:2509422] [WorldCat.org] [DOI] (P p)