Difference between revisions of "CitG"

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(Biological materials)
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|style="background:#ABCDEF;" align="center"|'''Function''' || TCA cycle
 
|style="background:#ABCDEF;" align="center"|'''Function''' || TCA cycle
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU33040 citG]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU33040 citG]
 
|-
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/CitG CitG]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/CitG CitG]
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yuxN]], [[yvzF]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yuxN]], [[yvzF]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15294&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU33040 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU33040 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU33040 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:citG_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:citG_context.gif]]

Revision as of 14:48, 13 May 2013

  • Description: fumarase

Gene name citG
Synonyms
Essential no
Product fumarate hydratase
Function TCA cycle
Gene expression levels in SubtiExpress: citG
Interactions involving this protein in SubtInteract: CitG
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 50 kDa, 5.475
Gene length, protein length 1386 bp, 462 aa
Immediate neighbours yuxN, yvzF
Sequences Protein DNA Advanced_DNA
Genetic context
CitG context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
CitG expression.png















Categories containing this gene/protein

carbon core metabolism

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU33040

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: (S)-malate = fumarate + H2O (according to Swiss-Prot)
  • Protein family: Fumarase subfamily (according to Swiss-Prot)
  • Paralogous protein(s): AnsB

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 3R6Q (aspartase from Bacillus sp. YM55-1, 43% identity, 77% similarity) PubMed
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation: constitutive
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Expression vector:
  • GFP fusion: GP1433 (spc, based on pGP1870), available in the Stülke lab
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed

Frederik M Meyer, Jan Gerwig, Elke Hammer, Christina Herzberg, Fabian M Commichau, Uwe Völker, Jörg Stülke
Physical interactions between tricarboxylic acid cycle enzymes in Bacillus subtilis: evidence for a metabolon.
Metab. Eng.: 2011, 13(1);18-27
[PubMed:20933603] [WorldCat.org] [DOI] (I p)

V A Price, I M Feavers, A Moir
Role of sigma H in expression of the fumarase gene (citG) in vegetative cells of Bacillus subtilis 168.
J. Bacteriol.: 1989, 171(11);5933-9
[PubMed:2509423] [WorldCat.org] [DOI] (P p)

K M Tatti, H L Carter, A Moir, C P Moran
Sigma H-directed transcription of citG in Bacillus subtilis.
J. Bacteriol.: 1989, 171(11);5928-32
[PubMed:2509422] [WorldCat.org] [DOI] (P p)

I M Feavers, V Price, A Moir
The regulation of the fumarase (citG) gene of Bacillus subtilis 168.
Mol. Gen. Genet.: 1988, 211(3);465-71
[PubMed:3130545] [WorldCat.org] (P p)

J S Miles, J R Guest
Complete nucleotide sequence of the fumarase gene (citG) of Bacillus subtilis 168.
Nucleic Acids Res.: 1985, 13(1);131-40
[PubMed:3923430] [WorldCat.org] [DOI] (P p)