Difference between revisions of "CitB"

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(Database entries)
(Database entries)
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=== Database entries ===
 
=== Database entries ===
  
* '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=8ACN 8ACN] mitochondrial aconitase (bos taurus)
+
* '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1L5J 1L5J] (''E. coli'')
  
 
* '''Swiss prot entry:'''
 
* '''Swiss prot entry:'''

Revision as of 17:26, 13 February 2009

  • Description: aconitate hydratase (aconitase)

Gene name citB
Synonyms
Essential no
Product aconitate hydratase (aconitase)
Function TCA cycle
MW, pI 99 kDa, 4.903
Gene length, protein length 2727 bp, 909 aa
Immediate neighbours sspO, yneN
Gene sequence (+200bp) Protein sequence
Genetic context
CitB context.gif



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

glutamate auxotrophy and a defect in sporulation PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: : Citrate <---> Isocitrate, binding to iron responsive elements (IRE RNA) in the absence of the FeS cluster
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s): FeS cluster
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure: 1L5J (E. coli)
  • Swiss prot entry:
  • KEGG entry:
  • E.C. number: 4.2.1.3

Additional information

B. subtilis aconitase is both an enzyme and an RNA binding protein PubMed

Expression and regulation

  • Regulation: repression by glucose + arginine (CcpC) PubMed
  • Additional information:

Biological materials

  • Mutant: GP683 (erm), available in Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Linc Sonenshein, Tufts University, Boston, MA, USA Homepage

Jörg Stülke, University of Göttingen, Germany Homepage

Your additional remarks

References

  1. Rosenkrantz MS, Dingman DW, Sonenshein AL (1985) Bacillus subtilis citB gene is regulated synergistically by glucose and glutamine. J Bacteriol 164:155-164. PubMed
  2. Jourlin-Castelli C, Mani N, Nakano MM, Sonenshein AL (2000) CcpC, a novel regulator of the LysR family required for glucose repression of the citB gene in Bacillus subtilis. J Mol Biol 295:865-878. PubMed
  3. Alén C, Sonenshein AL (1999) Bacillus subtilis aconitase is an RNA-binding protein. Proc Natl Acad Sci USA 96:10412-10417. PubMed
  4. Fisher SH, Magasanik B (1984) 2-Ketoglutarate and the regulation of aconitase and histidase formation Bacillus subtilis. J Bacteriol 158:379-382. PubMed
  5. Kim, H. J., S. I. Kim, M. Ratnayake-Lecamwasam, K. Tachikawa, A. L. Sonenshein, and M. Strauch. (2003) Complex regulation of the Bacillus subtilis aconitase gene. J. Bacteriol. 185:1672-1680. PubMed
  6. Blencke, H.-M., Reif, I., Commichau, F. M., Detsch, C., Wacker, I., Ludwig, H. & Stülke, J. (2006) Regulation of citB expression in Bacillus subtilis: Integration of multiple metabolic signals in the citrate pool and by the general nitrogen regulatory system. Arch. Microbiol. 185: 136-146. PubMed
  7. Serio, A. W., Pechter, K. B., and Sonenshein, A. L. (2006) Bacillus subtilis aconitase is required for efficient late-sporulation gene expression. J Bacteriol 188: 6396-6405. PubMed
  8. Nakano MM, Zuber P, Sonenshein AL (1998) Anaerobic regulation of Bacillus subtilis Krebs cycle genes. J Bacteriol. 180:3304-3311. PubMed
  9. Craig JE, Ford MJ, Blaydon DC, Sonenshein AL (1997) A null mutation in the Bacillus subtilis aconitase gene causes a block in Spo0A-phosphate-dependent gene expression. J Bacteriol 197:7351-7359. PubMed
  10. Kim HJ, Kim SI, Ratnayake-Lecamwasam M, Tachikawa K, Sonenshein AL, Strauch M (2003) Complex regulation of the Bacillus subtilis aconitase gene. J Bacteriol. 185:1672-1680. PubMed
  11. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed