Difference between revisions of "CdaS"

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(Biological materials)
(Biological materials)
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* '''His-tag fusion:'''
 
* '''His-tag fusion:'''
**pGP2591 10xHis-''cdaS'' under the control of native promoter (based on [[pAC7]]), available in [[Jörg Stülke]]'s lab**
+
**pGP2591 10xHis-''cdaS'' under the control of native promoter (based on [[pAC7]]), available in [[Jörg Stülke]]'s lab
**pGP2592 10xHis-''cdaS-L44F'' under the control of native promoter (based on [[pAC7]]), available in [[Jörg Stülke]]'s lab**
+
**pGP2592 10xHis-''cdaS-L44F'' under the control of native promoter (based on [[pAC7]]), available in [[Jörg Stülke]]'s lab
  
 
* '''two-hybrid system:'''  
 
* '''two-hybrid system:'''  

Revision as of 18:01, 13 August 2013

  • Description: sporulation-specific diadenylate cyclase, synthesis of c-di-AMP

Gene name cdaS
Synonyms yojJ
Essential no
Product sporulation-specific diadenylate cyclase
Function synthesis of c-di-AMP
Gene expression levels in SubtiExpress: cdaS
MW, pI 22 kDa, 5.367
Gene length, protein length 621 bp, 207 aa
Immediate neighbours yojK, yojI
Sequences Protein DNA DNA_with_flanks
Genetic context
YojJ context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YojJ expression.png















Categories containing this gene/protein

metabolism of signalling nucleotides, sporulation proteins

This gene is a member of the following regulons

SigG regulon

The gene

Basic information

  • Locus tag: BSU19430

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • synthesis of c-di-AMP from two molecules of ATP PubMed
  • Protein family:
  • Paralogous protein(s): CdaA

Extended information on the protein

  • Kinetic information:
  • Domains: contains a DAC domain involved in the synthesis of c-di-AMP PubMed
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 2FB5 (the protein of B. cereus, 48% identity, 81% similarity)
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • expressed late during sporulation in the forespore (SigG) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Expression vector:
    • IPTG inducible expression of B. cereus Strep-cdaS in E. coli: pGP2593 (in pGP172), available in Jörg Stülke's lab
  • lacZ fusion:
  • GFP fusion:
  • His-tag fusion:
    • pGP2591 10xHis-cdaS under the control of native promoter (based on pAC7), available in Jörg Stülke's lab
    • pGP2592 10xHis-cdaS-L44F under the control of native promoter (based on pAC7), available in Jörg Stülke's lab
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Ute Römling
Great times for small molecules: c-di-AMP, a second messenger candidate in Bacteria and Archaea.
Sci Signal: 2008, 1(33);pe39
[PubMed:18714086] [WorldCat.org] [DOI] (I e)

Original publications