Difference between revisions of "CdaS"

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|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''yojJ''
+
|''cdaS''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yojJ ''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
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|style="background:#ABCDEF;" align="center"|'''Function''' || synthesis of c-di-AMP
 
|style="background:#ABCDEF;" align="center"|'''Function''' || synthesis of c-di-AMP
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU19430 yojJ]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU19430 cdaS]
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 22 kDa, 5.367   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 22 kDa, 5.367   
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* '''Operon:'''  
 
* '''Operon:'''  
  
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yojJ_2118504_2119127_-1 yojJ] {{PubMed|22383849}}
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yojJ_2118504_2119127_-1 cdaS] {{PubMed|22383849}}
  
* '''[[Sigma factor]]:'''  
+
* '''[[Sigma factor]]:''' [[SigG]] {{PubMed|22383849}}
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
 +
** expressed late during sporulation in the forespore ([[SigG]]) {{PubMed|22383849}}
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
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<pubmed> 18714086</pubmed>
 
<pubmed> 18714086</pubmed>
 
==Original publications==
 
==Original publications==
<pubmed>21566650, 23192352 </pubmed>
+
<pubmed>21566650, 23192352 22383849</pubmed>
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 12:56, 30 November 2012

  • Description: sporulation-specific diadenylate cyclase, synthesis of c-di-AMP

Gene name cdaS
Synonyms yojJ
Essential no
Product sporulation-specific diadenylate cyclase
Function synthesis of c-di-AMP
Gene expression levels in SubtiExpress: cdaS
MW, pI 22 kDa, 5.367
Gene length, protein length 621 bp, 207 aa
Immediate neighbours yojK, yojI
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YojJ context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YojJ expression.png















Categories containing this gene/protein

metabolism of signalling nucleotides, sporulation proteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU19430

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • synthesis of c-di-AMP from two molecules of ATP PubMed
  • Protein family:
  • Paralogous protein(s): CdaA

Extended information on the protein

  • Kinetic information:
  • Domains: contains a DAC domain involved in the synthesis of c-di-AMP PubMed
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 2FB5 (the protein of B. cereus, 48% identity, 81% similarity)
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
    • expressed late during sporulation in the forespore (SigG) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Ute Römling
Great times for small molecules: c-di-AMP, a second messenger candidate in Bacteria and Archaea.
Sci Signal: 2008, 1(33);pe39
[PubMed:18714086] [WorldCat.org] [DOI] (I e)

Original publications

Felix M P Mehne, Katrin Gunka, Hinnerk Eilers, Christina Herzberg, Volkhard Kaever, Jörg Stülke
Cyclic di-AMP homeostasis in bacillus subtilis: both lack and high level accumulation of the nucleotide are detrimental for cell growth.
J. Biol. Chem.: 2013, 288(3);2004-17
[PubMed:23192352] [WorldCat.org] [DOI] (I p)

Pierre Nicolas, Ulrike Mäder, Etienne Dervyn, Tatiana Rochat, Aurélie Leduc, Nathalie Pigeonneau, Elena Bidnenko, Elodie Marchadier, Mark Hoebeke, Stéphane Aymerich, Dörte Becher, Paola Bisicchia, Eric Botella, Olivier Delumeau, Geoff Doherty, Emma L Denham, Mark J Fogg, Vincent Fromion, Anne Goelzer, Annette Hansen, Elisabeth Härtig, Colin R Harwood, Georg Homuth, Hanne Jarmer, Matthieu Jules, Edda Klipp, Ludovic Le Chat, François Lecointe, Peter Lewis, Wolfram Liebermeister, Anika March, Ruben A T Mars, Priyanka Nannapaneni, David Noone, Susanne Pohl, Bernd Rinn, Frank Rügheimer, Praveen K Sappa, Franck Samson, Marc Schaffer, Benno Schwikowski, Leif Steil, Jörg Stülke, Thomas Wiegert, Kevin M Devine, Anthony J Wilkinson, Jan Maarten van Dijl, Michael Hecker, Uwe Völker, Philippe Bessières, Philippe Noirot
Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis.
Science: 2012, 335(6072);1103-6
[PubMed:22383849] [WorldCat.org] [DOI] (I p)

Yaara Oppenheimer-Shaanan, Ezequiel Wexselblatt, Jehoshua Katzhendler, Eylon Yavin, Sigal Ben-Yehuda
c-di-AMP reports DNA integrity during sporulation in Bacillus subtilis.
EMBO Rep.: 2011, 12(6);594-601
[PubMed:21566650] [WorldCat.org] [DOI] (I p)