Difference between revisions of "CcpN"

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(Biological materials)
(Biological materials)
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* '''Mutant:'''  
* '''Mutant:'''  
**DB104 ''ccpN''::cat, available in [[Sabine Brantl]] lab
**DB104 ''ccpN''::cat, available in [[Sabine Brantl]] lab
**GP1128 ''ccpN''::cat, available in [[Stülke]] lab  
**GP1128 ''ccpN''::cat, available in [[Stülke]] lab  

Revision as of 08:53, 30 October 2012

  • Description: transcriptional repressor of gluconeogenetic genes and of sr1. repression in the presence of glucose

Gene name ccpN
Synonyms yqzB
Essential no
Product transcriptional regulator
Function repressor of genes involved in
gluconeogenesis (gapB, pckA) and of sr1
Gene expression levels in SubtiExpress: ccpN
Interactions involving this protein in SubtInteract: CcpN
Metabolic function and regulation of this protein in SubtiPathways:
Ammonium/ glutamate, Cys, Met & Sulfate assimilation, Central C-metabolism
MW, pI 23.4 kDa, 7.22
Gene length, protein length 636 bp, 212 amino acids
Immediate neighbours yqfL, glyS
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YqzB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
CcpN expression.png

Categories containing this gene/protein

carbon core metabolism, transcription factors and their control, regulators of core metabolism

This gene is a member of the following regulons

The CcpN regulon:

The gene

Basic information

  • Locus tag: BSU25250

Phenotypes of a mutant

Impaired growth on glucose due to re-routing of carbon from glycolysis to the pentose phosphate pathway PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: transcription repression of the gapB, pckA, and sr1 genes in the presence of glucose PubMed
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
    • YqfL (negative effector of CcpN activity) PubMed
    • ATP enhances CcpN-dependent repression, ADP counteracts the repressing activity of CcpN PubMed

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation: constitutively expressed PubMed
  • Additional information:
    • the intracellular concentration of CcpN is about 4 myM (according to PubMed).

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:

Labs working on this gene/protein

Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France

Sabine Brantl, Bacterial Genetics, Friedrich-Schiller-University of Jena, Germany homepage

Uwe Sauer, ETH Zürich, Switzerland homepage

Your additional remarks



Sabine Brantl, Andreas Licht
Characterisation of Bacillus subtilis transcriptional regulators involved in metabolic processes.
Curr. Protein Pept. Sci.: 2010, 11(4);274-91
[PubMed:20408793] [WorldCat.org] (I p)

Original Publications

Matthew L Ferguson, Dominique Le Coq, Matthieu Jules, Stéphane Aymerich, Ovidiu Radulescu, Nathalie Declerck, Catherine A Royer
Reconciling molecular regulatory mechanisms with noise patterns of bacterial metabolic promoters in induced and repressed states.
Proc. Natl. Acad. Sci. U.S.A.: 2012, 109(1);155-60
[PubMed:22190493] [WorldCat.org] [DOI] (I p)

Rita A Eckart, Sabine Brantl, Andreas Licht
Search for additional targets of the transcriptional regulator CcpN from Bacillus subtilis.
FEMS Microbiol. Lett.: 2009, 299(2);223-31
[PubMed:19732150] [WorldCat.org] [DOI] (I p)

Andreas Licht, Sabine Brantl
The transcriptional repressor CcpN from Bacillus subtilis uses different repression mechanisms at different promoters.
J. Biol. Chem.: 2009, 284(44);30032-8
[PubMed:19726675] [WorldCat.org] [DOI] (I p)

Simon Tännler, Eliane Fischer, Dominique Le Coq, Thierry Doan, Emmanuel Jamet, Uwe Sauer, Stéphane Aymerich
CcpN controls central carbon fluxes in Bacillus subtilis.
J. Bacteriol.: 2008, 190(18);6178-87
[PubMed:18586936] [WorldCat.org] [DOI] (I p)

Andreas Licht, Ralph Golbik, Sabine Brantl
Identification of ligands affecting the activity of the transcriptional repressor CcpN from Bacillus subtilis.
J. Mol. Biol.: 2008, 380(1);17-30
[PubMed:18511073] [WorldCat.org] [DOI] (I p)

Andreas Licht, Sabine Brantl
Transcriptional repressor CcpN from Bacillus subtilis compensates asymmetric contact distribution by cooperative binding.
J. Mol. Biol.: 2006, 364(3);434-48
[PubMed:17011578] [WorldCat.org] [DOI] (P p)

Andreas Licht, Sven Preis, Sabine Brantl
Implication of CcpN in the regulation of a novel untranslated RNA (SR1) in Bacillus subtilis.
Mol. Microbiol.: 2005, 58(1);189-206
[PubMed:16164558] [WorldCat.org] [DOI] (P p)

Pascale Servant, Dominique Le Coq, Stéphane Aymerich
CcpN (YqzB), a novel regulator for CcpA-independent catabolite repression of Bacillus subtilis gluconeogenic genes.
Mol. Microbiol.: 2005, 55(5);1435-51
[PubMed:15720552] [WorldCat.org] [DOI] (P p)