BrxA

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  • Description: disulfide isomerase, putative bacilliredoxin

Gene name yphP
Synonyms
Essential no
Product disulfide isomerase, putative bacilliredoxin
Function unknown
Gene expression levels in SubtiExpress: yphP
MW, pI 15 kDa, 4.619
Gene length, protein length 432 bp, 144 aa
Immediate neighbours ypiP, ilvD
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YphP context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YphP expression.png
























Categories containing this gene/protein

poorly characterized/ putative enzymes

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU21860

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: scpA family (according to Swiss-Prot)
  • Paralogous protein(s): yqiW

Extended information on the protein

  • Kinetic information:
  • Domains: contains an CxC redox motif [3]
  • Modification:
    • S-bacillithiolation upon hypochlorite stress on Cys-53 PubMed
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:

Database entries

  • KEGG entry: [4]
  • E.C. number:

Additional information

Expression and regulation

  • Operon: yphP (according to DBTBS)
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

YpdA, YqiW, YphP and YtxJ co-occur in those species predicted to synthesize bacillithiol.

References

Bui Khanh Chi, Alexandra A Roberts, Tran Thi Thanh Huyen, Katrin Bäsell, Dörte Becher, Dirk Albrecht, Chris J Hamilton, Haike Antelmann
S-bacillithiolation protects conserved and essential proteins against hypochlorite stress in firmicutes bacteria.
Antioxid Redox Signal: 2013, 18(11);1273-95
[PubMed:22938038] [WorldCat.org] [DOI] (I p)

Bui Khanh Chi, Katrin Gronau, Ulrike Mäder, Bernd Hessling, Dörte Becher, Haike Antelmann
S-bacillithiolation protects against hypochlorite stress in Bacillus subtilis as revealed by transcriptomics and redox proteomics.
Mol Cell Proteomics: 2011, 10(11);M111.009506
[PubMed:21749987] [WorldCat.org] [DOI] (I p)

Ahmed Gaballa, Gerald L Newton, Haike Antelmann, Derek Parsonage, Heather Upton, Mamta Rawat, Al Claiborne, Robert C Fahey, John D Helmann
Biosynthesis and functions of bacillithiol, a major low-molecular-weight thiol in Bacilli.
Proc Natl Acad Sci U S A: 2010, 107(14);6482-6
[PubMed:20308541] [WorldCat.org] [DOI] (I p)

Urszula Derewenda, Tomasz Boczek, Kelly L Gorres, Minmin Yu, Li-wei Hung, David Cooper, Andrzej Joachimiak, Ronald T Raines, Zygmunt S Derewenda
Structure and function of Bacillus subtilis YphP, a prokaryotic disulfide isomerase with a CXC catalytic motif .
Biochemistry: 2009, 48(36);8664-71
[PubMed:19653655] [WorldCat.org] [DOI] (I p)