Difference between revisions of "Bcd"

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=The gene=
 
=The gene=

Revision as of 16:58, 22 June 2009

  • Description: valine dehydrogenase, isoleucine dehydrogenase, L-leucine dehydrogenas

Gene name bcd
Synonyms bkd, yqiT
Essential no
Product valine dehydrogenase, isoleucine dehydrogenase,
L-leucine dehydrogenas
Function utilization of branched-chain keto acids
Metabolic function and regulation of this protein in SubtiPathways:
Ile, Leu, Val
MW, pI 39 kDa, 4.942
Gene length, protein length 1092 bp, 364 aa
Immediate neighbours buk, ptb
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
Bcd context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU24080

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: L-leucine + H2O + NAD+ = 4-methyl-2-oxopentanoate + NH3 + NADH (according to Swiss-Prot)
  • Protein family: Glu/Leu/Phe/Val dehydrogenases family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

M Nickel, G Homuth, C Böhnisch, U Mäder, T Schweder
Cold induction of the Bacillus subtilis bkd operon is mediated by increased mRNA stability.
Mol. Genet. Genomics: 2004, 272(1);98-107
[PubMed:15241682] [WorldCat.org] [DOI] (P p)

Tanja Kaan, Georg Homuth, Ulrike Mäder, Julia Bandow, Thomas Schweder
Genome-wide transcriptional profiling of the Bacillus subtilis cold-shock response.
Microbiology (Reading, Engl.): 2002, 148(Pt 11);3441-55
[PubMed:12427936] [WorldCat.org] [DOI] (P p)

M Debarbouille, R Gardan, M Arnaud, G Rapoport
Role of bkdR, a transcriptional activator of the sigL-dependent isoleucine and valine degradation pathway in Bacillus subtilis.
J. Bacteriol.: 1999, 181(7);2059-66
[PubMed:10094682] [WorldCat.org] (P p)