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  • Description: aminotransferase involved in bacilysin synthesis

Gene name bacF
Synonyms ywfG, ipa-85d
Essential no
Product aminotransferase
Function biosynthesis of the antibiotic bacilysin
Gene expression levels in SubtiExpress: bacF
MW, pI 44 kDa, 5.347
Gene length, protein length 1197 bp, 399 aa
Immediate neighbours bacG, bacE
Sequences Protein DNA DNA_with_flanks
Genetic context
YwfG context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
BacF expression.png

Categories containing this gene/protein

miscellaneous metabolic pathways, biosynthesis of antibacterial compounds

This gene is a member of the following regulons

AbrB regulon, CodY regulon, ScoC regulon

The gene

Basic information

  • Locus tag: BSU37690

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: YwfG catalyzes transamination of tetrahydro-4-hydroxyphenylpyruvate (with L-Phe as amino donor), to form tetrahydrotyrosine PubMed
  • Protein family: class-I pyridoxal-phosphate-dependent aminotransferase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Regulation:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks


Takashi Inaoka, Kozo Ochi
Scandium stimulates the production of amylase and bacilysin in Bacillus subtilis.
Appl Environ Microbiol: 2011, 77(22);8181-3
[PubMed:21948839] [] [DOI] (I p)

Türkan Ebru Köroğlu, Ismail Oğülür, Seval Mutlu, Ayten Yazgan-Karataş, Gülay Ozcengiz
Global regulatory systems operating in Bacilysin biosynthesis in Bacillus subtilis.
J Mol Microbiol Biotechnol: 2011, 20(3);144-55
[PubMed:21709425] [] [DOI] (I p)

Sarah A Mahlstedt, Christopher T Walsh
Investigation of anticapsin biosynthesis reveals a four-enzyme pathway to tetrahydrotyrosine in Bacillus subtilis.
Biochemistry: 2010, 49(5);912-23
[PubMed:20052993] [] [DOI] (I p)

Takashi Inaoka, Guojun Wang, Kozo Ochi
ScoC regulates bacilysin production at the transcription level in Bacillus subtilis.
J Bacteriol: 2009, 191(23);7367-71
[PubMed:19801406] [] [DOI] (I p)

Ayten Yazgan Karataş, Seçil Cetin, Gülay Ozcengiz
The effects of insertional mutations in comQ, comP, srfA, spo0H, spo0A and abrB genes on bacilysin biosynthesis in Bacillus subtilis.
Biochim Biophys Acta: 2003, 1626(1-3);51-6
[PubMed:12697329] [] (P p)

Takashi Inaoka, Kosaku Takahashi, Mayumi Ohnishi-Kameyama, Mitsuru Yoshida, Kozo Ochi
Guanine nucleotides guanosine 5'-diphosphate 3'-diphosphate and GTP co-operatively regulate the production of an antibiotic bacilysin in Bacillus subtilis.
J Biol Chem: 2003, 278(4);2169-76
[PubMed:12372825] [] [DOI] (P p)