Difference between revisions of "BacF"

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|colspan="2" | '''Genetic context''' <br/> [[Image:ywfG_context.gif]]
|colspan="2" | '''Genetic context''' <br/> [[Image:ywfG_context.gif]]
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=bacF_3868287_3869486_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:bacF_expression.png|500px]]

Revision as of 12:31, 23 April 2012

  • Description: aminotransferase involved in bacilysin synthesis

Gene name bacF
Synonyms ywfG, ipa-85d
Essential no
Product aminotransferase
Function biosynthesis of the antibiotic bacilysin
MW, pI 44 kDa, 5.347
Gene length, protein length 1197 bp, 399 aa
Immediate neighbours ywfH, bacE
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YwfG context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
BacF expression.png

Categories containing this gene/protein

miscellaneous metabolic pathways, biosynthesis of antibacterial compounds

This gene is a member of the following regulons

AbrB regulon, CodY regulon, ScoC regulon

The gene

Basic information

  • Locus tag: BSU37690

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: YwfG catalyzes transamination of tetrahydro-4-hydroxyphenylpyruvate (with L-Phe as amino donor), to form tetrahydrotyrosine PubMed
  • Protein family: class-I pyridoxal-phosphate-dependent aminotransferase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Regulation:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks


Additional publications: PubMed

Takashi Inaoka, Kozo Ochi
Scandium stimulates the production of amylase and bacilysin in Bacillus subtilis.
Appl Environ Microbiol: 2011, 77(22);8181-3
[PubMed:21948839] [WorldCat.org] [DOI] (I p)

Sarah A Mahlstedt, Christopher T Walsh
Investigation of anticapsin biosynthesis reveals a four-enzyme pathway to tetrahydrotyrosine in Bacillus subtilis.
Biochemistry: 2010, 49(5);912-23
[PubMed:20052993] [WorldCat.org] [DOI] (I p)

Takashi Inaoka, Guojun Wang, Kozo Ochi
ScoC regulates bacilysin production at the transcription level in Bacillus subtilis.
J Bacteriol: 2009, 191(23);7367-71
[PubMed:19801406] [WorldCat.org] [DOI] (I p)

Takashi Inaoka, Kosaku Takahashi, Mayumi Ohnishi-Kameyama, Mitsuru Yoshida, Kozo Ochi
Guanine nucleotides guanosine 5'-diphosphate 3'-diphosphate and GTP co-operatively regulate the production of an antibiotic bacilysin in Bacillus subtilis.
J Biol Chem: 2003, 278(4);2169-76
[PubMed:12372825] [WorldCat.org] [DOI] (P p)