yhfT

168

similar to long-chain fatty-acid-CoA ligase, involved in surfactin production

Locus: BSU_10360

Molecular weight: 52.71 kDa

Isoelectric point: 5.95

Protein length: 479 aa

Gene length: 1440 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: yhfT

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0318 (Galperin et al., 2021)

Gene

Coordinates: 1,110,479  1,111,918

The protein

Protein family

• ATP-dependent AMP-binding enzyme family (according to UniProt)

Structure

• 5BUQ (PDB) (MenE, 29% identitiy) PubMed
• O07619 (AlphaFold)

Paralogous protein(s)

• YngI, LcfA, LcfB

Expression and Regulation

Operons

• Genes: panU-yhfT-yhfS-yhfR

  Description: PubMed

  Regulation

  • repressed in the presence of biotin (BirA) PubMed

  Regulatory mechanism

  • BirA: repression, PubMed, in birA regulon

  Additional information

  • weakly expressed PubMed

• Genes: yhfT-yhfS-yhfR

  Description: PubMed

  Additional information

  • weakly expressed PubMed

Biological materials

Mutant

• MGNA-B280 (yhfT::erm), available at the NBRP B. subtilis, Japan
• available in Mohamed Marahiel's lab PubMed
• BKE10360 (yhfT::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GGTATGAGTAATTGTCATGT,  downstream forward: _UP4_CTGGAAGAGAGTGTACAGTA
• BKK10360 (yhfT::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GGTATGAGTAATTGTCATGT,  downstream forward: _UP4_CTGGAAGAGAGTGTACAGTA

References

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