yxiM

168

similar to rhamnogalacturonan acetylesterase

locus: BSU_39120

Molecular weight: 41.68 kDa

pI: 6.62

Protein length: 382 aa

Gene length: 1149 bp

function: unknown

product: unknown

essential: no

synonyms: yxiM

List of homologs in different organisms, belongs to COG3401 (Galperin et al., 2021)

Gene

Coordinates: 4,017,508 → 4,018,656

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The protein

Protein family

• [wiki|'GDSL' lipolytic enzyme family] (according to UniProt)

Structure

• [PDB|2O14]
• [AF|P42304]

Paralogous protein(s)

• [protein|578B79EB64B804FCAACA73AB22458481371A815C|yesY], [protein|585CA3154C0405697C4707E5B1D9359963999C54|yesT]

Expression and Regulation

Operons

• genes: [gene|D2790591E8C3B86BF91F6E1B6DADBA306D5EF758|yxiM]-[gene|EACD0C84AA3CE41EF05EAA3D25AA1153293EB562|deaD]

  description: [Pubmed|10746760]

  regulation

  • expression declines in the stationary phase [Pubmed|23175651]

Biological materials

Mutant

• MGNA-B719 (yxiM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1718 NBRP B. subtilis, Japan]
• GP1116 (spc), available in [wiki|Stülke] lab
• BKE39120 (Δ[gene|D2790591E8C3B86BF91F6E1B6DADBA306D5EF758|yxiM]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE39120 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCAAGACTCCCCCTCTAT,  downstream forward: _UP4_TAATACATCATTTTTCGACA
• BKK39120 (Δ[gene|D2790591E8C3B86BF91F6E1B6DADBA306D5EF758|yxiM]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK39120 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCAAGACTCCCCCTCTAT,  downstream forward: _UP4_TAATACATCATTTTTCGACA