yxiD

168

LXG toxin, non-specific metal-dependent RNase

locus: BSU_39300

Molecular weight: 64.13 kDa

pI: 9.4

Protein length: 569 aa

Gene length: 1710 bp

function: competetion with other bacteria in biofilms

product: LXG toxin, RNase

essential: no

ec: null

synonyms: yxiD

List of homologs in different organisms, belongs to COG5444 (Galperin et al., 2021)

Gene

Coordinates: 4,036,784 → 4,038,493

Phenotypes of a mutant

• the mutant is outcompeted by wild type cells in biofilms [pubmed|34280190]

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The protein

[wiki|Domains]

• [wiki|LXG domain] (aa 1-235) (according to UniProt)

Structure

• [AF|P42296]

[wiki|Localization]

• secreted
• secretion and delivery requires [protein|50D2A03E2D7B5D461540FD157377E0D37F771888|WXG100] and the T7SS ([protein|3CC3E197848E9688413C15CEA7DDCF0A7120A920|yukD]-[protein|3FE4A91C7D0B43C8704391F2F9493852B3AED9B0|essB]-[wiki|YukB]-[wiki|YueB]-[wiki|YueC]-[protein|B258CA26B0EC2310475FCCD67F1F196857FC0004|yueD]) [pubmed|34280190]

Expression and Regulation

Operons

• genes: [gene|75B0239578C92D4F3B298CBB077635BF5567C590|yxiB]-[gene|F44437883F39E5240D36340A7DD07803C5090ABB|yxiC]-[gene|BD8C0563C7123B6DEC1EEB602BDF2C9D92D34991|yxiD]-[gene|0DAFBDDC5E41592D06F97D404E75AF14B6783729|yxxD]-[gene|2710D9FD5FA50E09B4E482C13140F69B13C71861|yxxE]

  description: [Pubmed|22383849,7883710,10746760]

Biological materials

Mutant

• MGNA-B717 (yxiD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1716 NBRP B. subtilis, Japan]
• BKE39300 (Δ[gene|BD8C0563C7123B6DEC1EEB602BDF2C9D92D34991|yxiD]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE39300 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATCCCTTGTCCTCCCGCG,  downstream forward: _UP4_AAATGAGTTATAATTTTATA
• BKK39300 (Δ[gene|BD8C0563C7123B6DEC1EEB602BDF2C9D92D34991|yxiD]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK39300 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATCCCTTGTCCTCCCGCG,  downstream forward: _UP4_AAATGAGTTATAATTTTATA

References

• 7883710,10746760,22200572,34280190,36154281