yxeI

168

penicillin acylase

locus: BSU_39540

Molecular weight: 37.04 kDa

pI: 5.28

Protein length: 328 aa

Gene length: 987 bp

function: resistance to penicillin

product: penicillin acylase

essential: no

ec: null

synonyms: yxeI

List of homologs in different organisms, belongs to COG3049 (Galperin et al., 2021)

Gene

Coordinates: 4,062,543 → 4,063,529

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The protein

Catalyzed reaction/ biological activity

• acylation of penicillin V [Pubmed|21978958]

Protein family

• peptidase C59 family (single member, according to UniProt)

Structure

• [PDB|2OQC]
• [AF|P54948]

Expression and Regulation

Operons

• genes: [gene|B5153BCBEFFEA973B99F8B54047EF6FD830CAEE8|yxeI]-[gene|2976D8A6F6F40BA3FF226CF7B67F25DD237373D1|yxeJ]

  description: [Pubmed|10746760]

Biological materials

Mutant

• MGNA-B708 (yxeI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1707 NBRP B. subtilis, Japan]
• BKE39540 (Δ[gene|B5153BCBEFFEA973B99F8B54047EF6FD830CAEE8|yxeI]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE39540 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTGATCAGTCCCCTTTT,  downstream forward: _UP4_ATTCATGAGCTTAATTAATT
• BKK39540 (Δ[gene|B5153BCBEFFEA973B99F8B54047EF6FD830CAEE8|yxeI]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK39540 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTGATCAGTCCCCTTTT,  downstream forward: _UP4_ATTCATGAGCTTAATTAATT

References

• 10746760,15937167,21978958