dltX

168

required for D-alanylation of lipoteichoic acid (transfer of D-alanine from [protein|1B9BAEBE029BE9D4AD7E49C38B1E6DB5E8F4D4D3|dltB] to [protein|D178EC8A1FF22C99C437E01FC4843C5176564992|dltD])

locus: BSU_38499

Molecular weight: 5.90 kDa

pI: 7.899

Protein length: 49 aa

Gene length: 150 bp

function: D-alanylation of lipoteichoic acid

product: membrane microprotein

essential: -

synonyms: ywzH

List of homologs in different organisms

Gene

Coordinates: 3,952,108  3,952,257

Advertisement

The protein

Catalyzed reaction/ biological activity

• interacts with both [protein|1B9BAEBE029BE9D4AD7E49C38B1E6DB5E8F4D4D3|dltB] and [protein|D178EC8A1FF22C99C437E01FC4843C5176564992|dltD] and facilitates the interaction between those proteins [pubmed|37308592]
• involved in D-alanine transfer from [protein|1B9BAEBE029BE9D4AD7E49C38B1E6DB5E8F4D4D3|dltB] to [protein|D178EC8A1FF22C99C437E01FC4843C5176564992|dltD] [pubmed|37308592]

Structure

• [AF|C0H3T3]

[wiki|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|459ED28A98F4EC7E8A9016C742DC8EB5C26B5E65|dltX]-[gene|795CD64B65D7CBF26E5F706C7617196CC6FCF864|dltA]-[gene|1B9BAEBE029BE9D4AD7E49C38B1E6DB5E8F4D4D3|dltB]-[gene|862D977CF1E185597329A1EF0915485F72482A03|dltC]-[gene|D178EC8A1FF22C99C437E01FC4843C5176564992|dltD]-[gene|2FB3F09DA60A40212FE20217A64DAE714AB807CF|dltE]

  description: [Pubmed|29794222]

  regulation

  • expression is reduced in a [protein|D04629D798E458AD538809BB2B150B7FA81D2C7E|sigV] mutant [Pubmed|21926231]
  • the mRNA is processed between [gene|459ED28A98F4EC7E8A9016C742DC8EB5C26B5E65|dltX] and [gene|795CD64B65D7CBF26E5F706C7617196CC6FCF864|dltA] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|ricA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|ricF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|ricT] complex [Pubmed|29794222]

  regulatory mechanism

  • [protein|7098319D8E88FDC2A629A3F4A21507FD7A649385|yvrHb]: activation, [Pubmed|16306698], in [regulon|protein:7098319D8E88FDC2A629A3F4A21507FD7A649385|yvrHb regulon]
  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]: repression, [Pubmed|7797557], in [regulon|protein:2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A regulon]
  • stringent response: negative regulation, in [regulon|other_regulator:stringent response|stringent response]

  sigma factors

  • [protein|7024E4162A6D827069F882FDEACA696EBC05DD40|sigD]: sigma factor, [Pubmed|7797557], in [regulon|protein:7024E4162A6D827069F882FDEACA696EBC05DD40|sigD regulon]
  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]: sigma factor, [Pubmed|18179421], in [regulon|protein:081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM regulon]
  • [protein|E77364F274A520FCCA9F5C9504E317B047BA29E6|sigX]: sigma factor, [Pubmed|14762009], in [regulon|protein:E77364F274A520FCCA9F5C9504E317B047BA29E6|sigX regulon]
  • [protein|D04629D798E458AD538809BB2B150B7FA81D2C7E|sigV]: sigma factor, [Pubmed|21926231], in [regulon|protein:D04629D798E458AD538809BB2B150B7FA81D2C7E|sigV regulon]

Biological materials

Mutant

• BKE38499 ([gene|459ED28A98F4EC7E8A9016C742DC8EB5C26B5E65|dltX]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE38499 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATCAAAAATTCCCTTCC,  downstream forward: _UP4_TTCTAATTGGAGAGAGAATA
• BKK38499 ([gene|459ED28A98F4EC7E8A9016C742DC8EB5C26B5E65|dltX]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK38499 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATCAAAAATTCCCTTCC,  downstream forward: _UP4_TTCTAATTGGAGAGAGAATA

References

Reviews

• 36882621

Original Publications

• 29794222,37308592,28824570