yvrE

168

glucono-lactonase, general stress protein, similar to senescence marker protein-30

locus: BSU_33200

Molecular weight: 33.05 kDa

pI: 4.62

Protein length: 292 aa

Gene length: 879 bp

function: survival of salt stress

product: glucono-lactonase

essential: no

synonyms: yvrE

List of homologs in different organisms, belongs to COG3386 (Galperin et al., 2021)

Gene

Coordinates: 3,405,664  3,406,542

Advertisement

The protein

Catalyzed reaction/ biological activity

• glucoco-lactonase [pubmed|29379263]

Protein family

• SMP-30/CGR1 family (single member, according to UniProt)

Structure

• [PDB|3G4E] (the human protein, 33% identity) [pubmed|20329768]
• [AF|O34940]

Expression and Regulation

Operons

• genes: [gene|0F65F634368E935585FC015BA64085F3A2244F9B|yvrE]

  description: [Pubmed|16306698]

  regulation

  • induced by stress ([protein|search|SigB]) [Pubmed|15805528]

  sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [Pubmed|15805528], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]

• genes: [gene|7098319D8E88FDC2A629A3F4A21507FD7A649385|yvrHb]-[gene|96E4470EF4B558F4EC318FEBF58B779BA50DB7E2|yvrG]-[gene|0F65F634368E935585FC015BA64085F3A2244F9B|yvrE]

  description: [Pubmed|22383849]

additional information

• translation is substantially enhanced when the gene is transcribed by [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]-[wiki|RNA polymerase] [pubmed|33927010]

Biological materials

Mutant

• MGNA-B048 (yvrE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1047 NBRP B. subtilis, Japan]
• BKE33200 ([gene|0F65F634368E935585FC015BA64085F3A2244F9B|yvrE]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE33200 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTGTTACACCTCTTTAG,  downstream forward: _UP4_TAAAAAAAACGCATCCGAAA
• BKK33200 ([gene|0F65F634368E935585FC015BA64085F3A2244F9B|yvrE]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK33200 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTGTTACACCTCTTTAG,  downstream forward: _UP4_TAAAAAAAACGCATCCGAAA

References

• 15805528,16306698,29379263,20329768,33927010