YvcK

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  • Description: essential for growth under gluconeogenetic conditions, required for correct localization of PBP1

Gene name yvcK
Synonyms
Essential no
Product unknown
Function localization of PBP1
Gene expression levels in SubtiExpress: yvcK
Interactions involving this protein in SubtInteract: YvcK
MW, pI 34 kDa, 5.408
Gene length, protein length 951 bp, 317 aa
Immediate neighbours yvcL, yvcJ
Sequences Protein DNA DNA_with_flanks
Genetic context
YvcK context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YvcK expression.png

Contents















Categories containing this gene/protein

carbon core metabolism, cell shape, phosphoproteins

This gene is a member of the following regulons

Efp-dependent proteins

The gene

Basic information

  • Locus tag: BSU34760

Phenotypes of a mutant

  • unable to grow with gluconeogenic substrates as single carbon source PubMed
  • filamentous or L-shape-like aberrant morphologies (suppressed by Mg++) PubMed, this is supressed by overexpression of MreB or by deletion of ponA (PBP1 ) PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: UPF0052 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 2O2Z (the protein from B. halodurans, 63% identity, 86% similarity)
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: very weak stimuation of expression by citrate and succinate PubMed
  • Regulatory mechanism:
  • Additional information:
    • translation is likely to require Efp due to the presence of several consecutive proline residues PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 96 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 246 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 555 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 282 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 459 PubMed

Biological materials

  • Mutant:
  • Expression vector: pGP736 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Stülke lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Görke lab
  • Antibody:

Labs working on this gene/protein

Boris Görke, University of Göttingen, Germany Homepage

Anne Galinier, University of Marseille, France

Your additional remarks

References

Reviews

Johann Mignolet, Patrick H Viollier
A sweet twist gets Bacillus into shape.
Mol. Microbiol.: 2011, 80(2);283-5
[PubMed:21371139] [WorldCat.org] [DOI] (I p)

Original publications

Mushtaq Mir, Sladjana Prisic, Choong-Min Kang, Shichun Lun, Haidan Guo, Jeffrey P Murry, Eric J Rubin, Robert N Husson
Mycobacterial gene cuvA is required for optimal nutrient utilization and virulence.
Infect. Immun.: 2014, 82(10);4104-17
[PubMed:25047842] [WorldCat.org] [DOI] (I p)

Elodie Foulquier, Frédérique Pompeo, Céline Freton, Baptiste Cordier, Christophe Grangeasse, Anne Galinier
PrkC-mediated phosphorylation of overexpressed YvcK protein regulates PBP1 protein localization in Bacillus subtilis mreB mutant cells.
J. Biol. Chem.: 2014, 289(34);23662-9
[PubMed:25012659] [WorldCat.org] [DOI] (I p)

Elodie Foulquier, Frédérique Pompeo, Alain Bernadac, Leon Espinosa, Anne Galinier
The YvcK protein is required for morphogenesis via localization of PBP1 under gluconeogenic growth conditions in Bacillus subtilis.
Mol. Microbiol.: 2011, 80(2);309-18
[PubMed:21320184] [WorldCat.org] [DOI] (I p)

Boris Görke, Elodie Foulquier, Anne Galinier
YvcK of Bacillus subtilis is required for a normal cell shape and for growth on Krebs cycle intermediates and substrates of the pentose phosphate pathway.
Microbiology (Reading, Engl.): 2005, 151(Pt 11);3777-91
[PubMed:16272399] [WorldCat.org] [DOI] (P p)

A Galinier, J Haiech, M C Kilhoffer, M Jaquinod, J Stülke, J Deutscher, I Martin-Verstraete
The Bacillus subtilis crh gene encodes a HPr-like protein involved in carbon catabolite repression.
Proc. Natl. Acad. Sci. U.S.A.: 1997, 94(16);8439-44
[PubMed:9237995] [WorldCat.org] (P p)

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