yukE

168

secreted protein of the WXG100 superfamily, homolog of virulence factor EsxA

locus: BSU_31910

Molecular weight: 9.00 kDa

pI: 7.91

Protein length: 97 aa

Gene length: 294 bp

function: secretion and delivery of [wiki|LXG domain] toxins

product: [wiki|LXG domain] toxin delivery factor

essential: no

synonyms: yukE

List of homologs in different organisms, belongs to COG4842 (Galperin et al., 2021)

Gene

Coordinates: 3,276,141 3,276,434

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The protein

Catalyzed reaction/ biological activity

• secretion and delivery of [wiki|LXG domain] toxins [pubmed|34280190]

Protein family

• WXG100 family (with [protein|A177DF098115F85F23A1D42EFB07D294B515830F|yfjA], according to UniProt) (pfam06013) [Pubmed|11973144]

Structure

• [PDB|3ZBH] (from Geobacillus thermodenitrificans, 59% identity)
• [AF|C0SP85]

[wiki|Localization]

• secreted, by the type VII [wiki|protein secretion] system [protein|3CC3E197848E9688413C15CEA7DDCF0A7120A920|yukD]-[protein|3FE4A91C7D0B43C8704391F2F9493852B3AED9B0|essB]-[protein|6007664F3D979B4D8D4662C7F6E5CF2F489268E3|yukB]-[protein|0A38B2E900532C9950DE6E629F5EB21D64E18B55|yueB]-[protein|DA52C4CD130F32734E01A52F2F395127D2260F61|yueC] [Pubmed|24828531,23861817]

Additional information

• belongs to the 100 most abundant proteins [PubMed|15378759]

Expression and Regulation

Operons

• genes: [gene|50D2A03E2D7B5D461540FD157377E0D37F771888|yukE]

  description: [Pubmed|22383849]

• genes: [gene|50D2A03E2D7B5D461540FD157377E0D37F771888|yukE]-[gene|3CC3E197848E9688413C15CEA7DDCF0A7120A920|yukD]-[gene|3FE4A91C7D0B43C8704391F2F9493852B3AED9B0|essB]-[gene|6007664F3D979B4D8D4662C7F6E5CF2F489268E3|yukB]-[gene|0A38B2E900532C9950DE6E629F5EB21D64E18B55|yueB]-[gene|DA52C4CD130F32734E01A52F2F395127D2260F61|yueC]-[gene|B258CA26B0EC2310475FCCD67F1F196857FC0004|yueD]

  description: [Pubmed|22383849]

  regulation

  • expressed in the stationary phase [Pubmed|23861817]
  • expression is reduced in motile cells as compared to non-motile cells [pubmed|33782055]

  regulatory mechanism

  • [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|degU]: activation, [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|degU]-P, [Pubmed|23861817], in [regulon|protein:D343D2096664A972026D911E7A17A35C7B1CD1C9|degU regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|22383849], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

Biological materials

Mutant

• MGNA-A628 (yukE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/628 NBRP B. subtilis, Japan]
• BKE31910 ([gene|50D2A03E2D7B5D461540FD157377E0D37F771888|yukE]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE31910 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTCCTCATTACCTCCT, downstream forward: _UP4_TAACAGTATGAAAGGGAAGG
• BKK31910 ([gene|50D2A03E2D7B5D461540FD157377E0D37F771888|yukE]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK31910 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTCCTCATTACCTCCT, downstream forward: _UP4_TAACAGTATGAAAGGGAAGG

References

• 15576783,22383849,11973144,23861817,23861817,15378759,24798022,24696501,21395229,24828531,33782055,34280190,34521822,36154281