ytxM

168

similar to prolyl aminopeptidase

locus: BSU_30810

Molecular weight: 30.55 kDa

pI: 7.18

Protein length: 274 aa

Gene length: 825 bp

function: ubiquinone and menaquinone biosynthesis

product: unknown

essential: no

ec: 4.2.99.20

synonyms: ytxM, ytfB

List of homologs in different organisms, belongs to COG0596 (Galperin et al., 2021)

Gene

Coordinates: 3,149,751  3,150,575

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The protein

Catalyzed reaction/ biological activity

• 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate --> (1R,6R)-6-hydroxy-2-succinyl-cyclohexa-2,4-diene-1-carboxylate + pyruvate (according to UniProt)

Protein family

• [wiki|AB hydrolase superfamily] (according to UniProt)

[wiki|Domains]

• [wiki|AB hydrolase-1 domain] (aa 26-259) (according to UniProt)

Structure

• [PDB|2XMZ] (from ''Staphylococcus aureus'', 34% identity) [Pubmed|21513522]
• [AF|P23974]

Expression and Regulation

Operons

• genes: [gene|8EFE4D2B07E54A0EE7D6821C9FEBFC7DC22FDE03|menF]-[gene|E3283812B7EB374EAC8722ECCE28432DE24B7B4C|menD]-[gene|74659B6794496912E139B3CF9EFF787B63729A3F|ytxM]-[gene|224584953B0BC1AF666CD69372B2D53AC56F6752|menB]-[gene|16412BAB04BBF9FD1C3653328923687874C81868|menE]-[gene|9B2F45BC37D4F356B366447A33DC95639EF484EE|menC]

  description: [Pubmed|9387221,1629163,8550504]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8550504], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

  additional information

  • A [protein|search|ncRNA] is predicted between '[protein|search|mntA]' and '[protein|search|menC]' [PubMed|20525796]

Biological materials

Mutant

• MGNA-A283 (ytxM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/283 NBRP B. subtilis, Japan]
• BKE30810 ([gene|74659B6794496912E139B3CF9EFF787B63729A3F|ytxM]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE30810 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GACACCATCTGATACCGTTA,  downstream forward: _UP4_TGACTCATTCACATAGAGAA
• BKK30810 ([gene|74659B6794496912E139B3CF9EFF787B63729A3F|ytxM]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK30810 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GACACCATCTGATACCGTTA,  downstream forward: _UP4_TGACTCATTCACATAGAGAA

References

• 10913081,9387221,1629163,8550504,12454479,21513522