facZ

168

Firmicute-associated coordinator of Z-rings, general stress protein

locus: BSU_29780

Molecular weight: 15.60 kDa

pI: 9.98

Protein length: 140 aa

Gene length: 423 bp

function: control of division site placement

product: coordinator of Z-rings

essential: no

synonyms: ytxG, csb40, facZ

List of homologs in different organisms, belongs to COG4768 (Galperin et al., 2021)

Gene

Coordinates: 3,047,593  3,048,015

Phenotypes of a mutant

• [category|SW.1.1.8|Cell division] and membrane defects [pubmed|38443581]
• inactivation of ''[gene|00A42FDB559BE2647CD682180420A435E2E7035F|facZ]'' reduces sporulation efficiency to 39.6% that of wild type cells; aberrant membrane morphologies [Pubmed|26735940]

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The protein

Protein family

• UPF0478 family (single member, according to UniProt)

Structure

• [AF|P40779]

[wiki|Localization]

• membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|00A42FDB559BE2647CD682180420A435E2E7035F|facZ]-[gene|43BF293F735ED783D36F33AA4701D57F2AED6657|ytxH]-[gene|8B4E1D087B75B5E77BCB60FF3465270CEB72B028|brxC]

  description: [Pubmed|8733232]

  regulation

  • induced by stress ([protein|search|SigB]) [Pubmed|8733232,11544224]

  sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [Pubmed|8733232,11544224], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]
  • [protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH]: sigma factor, [Pubmed|8733232], in [regulon|protein:DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH regulon]

Biological materials

Mutant

• MGNA-B529 (ytxG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1528 NBRP B. subtilis, Japan]
• BKE29780 ([gene|00A42FDB559BE2647CD682180420A435E2E7035F|facZ]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE29780 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTCAAACCTCCTTGCTG,  downstream forward: _UP4_TAAGAGAAAAAAATACTAAG
• BKK29780 ([gene|00A42FDB559BE2647CD682180420A435E2E7035F|facZ]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK29780 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTCAAACCTCCTTGCTG,  downstream forward: _UP4_TAAGAGAAAAAAATACTAAG

References

• 11544224,8733232,18763711,26735940,38443581