cmoJ

cmoJ
168

flavin-dependent monooxygenase, required for the conversion of S-methyl-cysteine to cysteine

locus
BSU_29310
Molecular weight
49.25 kDa
pI
5.72
Protein length
Gene length
function
utilization of S-methyl-cysteine
product
flavin-dependent monooxygenase
essential
no
synonyms
cmoJ, ytnJ

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG2141 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,001,724  3,003,052
Phenotypes of a mutant
no growth with S-methyl cysteine [Pubmed|23944997]
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The protein
Catalyzed reaction/ biological activity
oxidation of S-methyl-cysteine [Pubmed|23944997]
conversion of N-acetyl-S-benzyl-l-cysteine sulfoxide to benzaldehyde and N-acetyl-l-cysteine sulfenic acid [pubmed|35584544]
Protein family
ntaA/snaA/soxA(dszA) monooxygenase family (with [protein|19E6EF9CCA31440EAC046F8D6CA98B6A08589D58|yxeK], according to UniProt)
[wiki|Cofactors]
FMN [pubmed|35584544]
Structure
[PDB|1YW1] (complex with FMN),  [PDB|1TVL], [PDB|6ASK], [PDB|6ASL]
[AF|O34974]
Paralogous protein(s)
[protein|19E6EF9CCA31440EAC046F8D6CA98B6A08589D58|yxeK]
Expression and Regulation
Operons
genes
[gene|E3F1B3A4086DBC40FD9AE9CD6981270FBDFA6CAF|snaA]-[gene|A6A13C54177381CE78ABA2CFC2A3F2CE12099F15|tcyJ]-[gene|A8FA40EACDB15CF53DDDA725A3AA5A6EB654FA8B|tcyK]-[gene|350C97E4A4C60A6E2FF9610D50B4A49FB9F9B42D|tcyL]-[gene|B1DD32A3B3D1C0C5391A40834940257357A2F30F|tcyM]-[gene|B24763A732111026D21C828FF9BE73FB22A123CF|tcyN]-[gene|EDC3565D90D7059A4A4C66A13EB568420F6CECD4|cmoO]-[gene|FCD25CA4414D689B217F54B0C44EB805F9590454|cmoI]-[gene|8145952307BC47805A7A9BD39418474358043BFE|cmoJ]-[gene|3D32B6C1DC9CAC615B02E14EA846648C3B0E61D6|ribR]-[gene|0DE66A10D9475DC57A140561E8532D3EC038FD6F|sndA]-[gene|910EAAF48C85D0364751EC9585121246AF677431|ytnM]
description
[Pubmed|15668000]
regulation
induced in the presence of methionine and taurine [Pubmed|11390694]
regulatory mechanism
[protein|50930C56C27D22715620A350220E3C56ADB41020|cymR]: repression, [Pubmed|16109943], in [regulon|protein:50930C56C27D22715620A350220E3C56ADB41020|cymR regulon]
[protein|741156D495BE3857683C8A0390764EAD83845ABC|ascR]: activation, [Pubmed|16109943], in [regulon|protein:741156D495BE3857683C8A0390764EAD83845ABC|ascR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|15272571], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
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[gene|E3F1B3A4086DBC40FD9AE9CD6981270FBDFA6CAF|snaA]→[gene|910EAAF48C85D0364751EC9585121246AF677431|ytnM]

2024-02-06 19:38:33

Jstuelk

61

8a3868e633778d2f68bf47d615f22f716e843294

4D6CA410A1DC104A491D3C18248CCD50CC0E983A

Biological materials
Mutant
MGNA-A155 (ytnJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/155 NBRP B. subtilis, Japan]
BKE29310 ([gene|8145952307BC47805A7A9BD39418474358043BFE|cmoJ]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE29310 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AAACTGAATAAAATCTGCAC,  downstream forward: _UP4_TAGGTTTCGTCTGATCGAAT
BKK29310 ([gene|8145952307BC47805A7A9BD39418474358043BFE|cmoJ]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK29310 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AAACTGAATAAAATCTGCAC,  downstream forward: _UP4_TAGGTTTCGTCTGATCGAAT
References
16109943,15668000,15272571,23944997,35584544

8145952307BC47805A7A9BD39418474358043BFE

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Time of last update: 2024-03-28 05:37:17

Author of last update: Jstuelk