rpmEB

168

general stress protein, binds in the stationary phase to the [wiki|ribosome], replaces [protein|035154A4160F084BA1CC124D0951937B203E167D|rpmE] under conditions of zinc limitation

locus: BSU_30700

Molecular weight: 9.39 kDa

pI: 9.81

Protein length: 82 aa

Gene length: 249 bp

function: survival of salt stress

product: accessory ribosomal protein

essential: no

synonyms: rpmEB, rpmE2, ytiA

List of homologs in different organisms, belongs to COG0254 (Galperin et al., 2021)

Gene

Coordinates: 3,138,978 → 3,139,226

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The protein

Protein family

• bacterial [wiki|ribosomal protein] bL31 family (with [protein|035154A4160F084BA1CC124D0951937B203E167D|rpmE], according to UniProt)

Structure

• [PDB|5O5J] (30S ribosome subunit of Mycobacterium smegmatis, 43% identity) [pubmed|28683309]
• [AF|O34967]

Modification

• phosphorylated on Arg-70 [Pubmed|22517742]

Paralogous protein(s)

• [protein|035154A4160F084BA1CC124D0951937B203E167D|rpmE]

Expression and Regulation

Operons

• genes: [gene|DAE7AB27A4C42BF50556BB76F33BEFA8DF35EA3A|rpmEB]

  description: [Pubmed|22383849]

  regulation

  • induced by stress ([protein|search|SigB]) [Pubmed|15805528]

  regulatory mechanism

  • [protein|A0CD8CCB54AE9F10DE3C876FB47B9877C303862D|zur]: repression, in the presence of zinc [PubMed|12904577,15049826], in [regulon|protein:A0CD8CCB54AE9F10DE3C876FB47B9877C303862D|zur regulon]

  sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [Pubmed|15805528], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]

Biological materials

Mutant

• MGNA-A292 (ytiA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/292 NBRP B. subtilis, Japan]
• BKE30700 (Δ[gene|DAE7AB27A4C42BF50556BB76F33BEFA8DF35EA3A|rpmEB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE30700 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGGGTATCTCCTTTCAAT,  downstream forward: _UP4_TAAGGCAGGCCTGAGGGTTT
• BKK30700 (Δ[gene|DAE7AB27A4C42BF50556BB76F33BEFA8DF35EA3A|rpmEB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK30700 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGGGTATCTCCTTTCAAT,  downstream forward: _UP4_TAAGGCAGGCCTGAGGGTTT

References

• 36815589,17163968,15049826,16547061,12904577,22383849,15805528,19648245,22517742,23002217,27561249,28683309