ythA

168

cytochrome bd2, menaquinol oxidase (7:1 protons)

locus: BSU_30710

Molecular weight: 48.49 kDa

pI: 9.42

Protein length: 443 aa

Gene length: 1332 bp

function: respiration

product: cytochrome bd2, menaquinol oxidase (7:1 protons)

essential: no

synonyms: ythA

List of homologs in different organisms, belongs to COG1271 (Galperin et al., 2021)

Gene

Coordinates: 3,139,431 → 3,140,762

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The protein

Protein family

• cytochrome ubiquinol oxidase subunit 1 family (with [protein|C0459C0D45E31561D281E016D86CD80DCAF13357|cydA], according to UniProt)

Structure

• [PDB|5DOQ] (from Geobacillus thermodenitrificans, 42% identity) [pubmed|27126043]
• [AF|C0SP90]

Paralogous protein(s)

• [protein|C0459C0D45E31561D281E016D86CD80DCAF13357|cydA]

[wiki|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|EB8AF295A11F28BDFAE39038FA89890824FC7791|ythA]-[gene|813E4BBEC8701114503ACD66DD6FE5A06B6B5E5F|ythB]-[gene|74DEE21BF65279A02A64EFCB60A06573F7C46B19|ytzL]

  description: [Pubmed|22383849]

  regulation

  • expressed during [wiki|sporulation] [Pubmed|22383849]

Biological materials

Mutant

• MGNA-A291 (ythA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/291 NBRP B. subtilis, Japan]
• BKE30710 (Δ[gene|EB8AF295A11F28BDFAE39038FA89890824FC7791|ythA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE30710 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACAATCGTAAACCACCTTT,  downstream forward: _UP4_TAATGGCTCCGCAAACAGGA
• BKK30710 (Δ[gene|EB8AF295A11F28BDFAE39038FA89890824FC7791|ythA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK30710 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACAATCGTAAACCACCTTT,  downstream forward: _UP4_TAATGGCTCCGCAAACAGGA

References

• 11073895,10551842,27126043