ytfP

168

putative NAD(FAD)-utilizing dehydrogenase

locus: BSU_30060

Molecular weight: 35.01 kDa

pI: 9.57

Protein length: 420 aa

Gene length: 1263 bp

function: unknown

product: unknown

essential: no

synonyms: ytfP

List of homologs in different organisms, belongs to COG2081 (Galperin et al., 2021)

Gene

Coordinates: 3,075,367  3,076,629

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The protein

Structure

• [PDB|2I0Z] (from B. cereus, 70% identity)
• [AF|Q795R8]

Expression and Regulation

Operons

• genes: [gene|86274AAF9E0D0882F1AD9D4A528BECB013B71C38|ytfP]-[gene|597B517CA4277E33153F80919213BFF7BE495563|opuD]

  description: [Pubmed|22383849]

  regulation

  • induced by cold stress [Pubmed|21296969]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|21296969], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [Pubmed|21296969], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]

Biological materials

Mutant

• MGNA-A816 (ytfP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/816 NBRP B. subtilis, Japan]
• BKE30060 ([gene|86274AAF9E0D0882F1AD9D4A528BECB013B71C38|ytfP]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE30060 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTTTATCATCAACTTTC,  downstream forward: _UP4_TAATCATTACTCTTTAAATC
• BKK30060 ([gene|86274AAF9E0D0882F1AD9D4A528BECB013B71C38|ytfP]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK30060 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTTTATCATCAACTTTC,  downstream forward: _UP4_TAATCATTACTCTTTAAATC