yrvC

168

unknown

locus: BSU_27640

Molecular weight: 18.20 kDa

pI: 4.87

Protein length: 165 aa

Gene length: 498 bp

function: unknown

product: unknown

essential: no

synonyms: yrvC

List of homologs in different organisms, belongs to COG0490 (Galperin et al., 2021)

Gene

Coordinates: 2,826,245  2,826,742

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The protein

[wiki|Domains]

• contains a [wiki|RCK_C domain] (aa 76-161) at the C-terminus (according to [http://www.uniprot.org/uniprot/?query=RCK+C-terminal+domain&sort=score UniProt])

Structure

• [AF|O32046]

Paralogous protein(s)

• [protein|D8A8FBC05B9665F72A88685D2B33822C2BB84C62|khtT]

Expression and Regulation

Operons

• genes: [gene|4AF865F1E16187F89C1FE19BAE5C2CF2C0A53E4A|yrvC]-[gene|418A95E9DD8DE2690C1E65E2A4198E5C5AC6E6D3|yrvD]

  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-B518 (yrvC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1517 NBRP B. subtilis, Japan]
• GP2099 ([gene|4AF865F1E16187F89C1FE19BAE5C2CF2C0A53E4A|yrvC]-[gene|418A95E9DD8DE2690C1E65E2A4198E5C5AC6E6D3|yrvD]::''zeo''), available in [wiki|Jörg Stülke]'s lab
• BKE27640 ([gene|4AF865F1E16187F89C1FE19BAE5C2CF2C0A53E4A|yrvC]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE27640 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATATAAACCCTCCTACA,  downstream forward: _UP4_TAACCTAGTGTTAAGCCATG
• BKK27640 ([gene|4AF865F1E16187F89C1FE19BAE5C2CF2C0A53E4A|yrvC]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK27640 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATATAAACCCTCCTACA,  downstream forward: _UP4_TAACCTAGTGTTAAGCCATG
• GP2713 ([gene|4AF865F1E16187F89C1FE19BAE5C2CF2C0A53E4A|yrvC]-[gene|418A95E9DD8DE2690C1E65E2A4198E5C5AC6E6D3|yrvD]::''cat''), available in [wiki|Jörg Stülke]'s lab

Expression vectors

• pGP2908 (N-terminal His-tag, purification from ''E. coli'', in [wiki|pWH844]), available in [wiki|Jörg Stülke]'s lab

References

Reviews

• 31361596