yrkH

168

unknown

locus: BSU_26510

Molecular weight: 32.73 kDa

pI: 4.85

Protein length: 377 aa

Gene length: 1134 bp

function: unknown

product: unknown

essential: no

synonyms: yrkH

List of homologs in different organisms, belongs to COG0607 (Galperin et al., 2021)

Gene

Coordinates: 2,711,109  2,712,242

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The protein

[wiki|Domains]

• C-terminal metallo--lactamase domain (according to UniProt)
• [wiki|Rhodanese domain] (aa 19-114) (according to UniProt)

Structure

• [PDB|3R2U] (metallo--lactamase from Staphylococcus aureus, corresponds to the C-terminal domain of [protein|06CC915A18E5ADD42B784B7BBBFD4C344559A34E|yrkH], aa 142 ... 353, 31% identity)
• [AF|P54435]

Expression and Regulation

Operons

• genes: [gene|6AD1DD074B2F044C6A4E4B98FABC4E7FD67B3139|yrzM]-[gene|8A3F1BFDF85DD96B7FB4EBC807F71639AF69DE98|yrkD]-[gene|1F1FE531622CD218EE8990CDAC6D2D2F9E86FE3F|yrkE]-[gene|5FC3DF596816AA4E8541B324EC6DE6D89C90C8E2|yrkF]-[gene|06CC915A18E5ADD42B784B7BBBFD4C344559A34E|yrkH]-[gene|D996F581E21395C3F9CC064800C49A765EFC17A7|yrkI]-[gene|F3E842844B315FE438F66995CDFB8BA1C32BDF23|yrkJ]-[gene|1BEBD09273240B6B50DABCB160FCCBC205BB2AAE|yrkK]-[gene|2EA9792C1C7CD89E43742F57FFB7300725B6AEF4|yrkL]

  description: [Pubmed|22383849]

• genes: [gene|6AD1DD074B2F044C6A4E4B98FABC4E7FD67B3139|yrzM]-[gene|8A3F1BFDF85DD96B7FB4EBC807F71639AF69DE98|yrkD]-[gene|1F1FE531622CD218EE8990CDAC6D2D2F9E86FE3F|yrkE]-[gene|5FC3DF596816AA4E8541B324EC6DE6D89C90C8E2|yrkF]-[gene|06CC915A18E5ADD42B784B7BBBFD4C344559A34E|yrkH]-[gene|D996F581E21395C3F9CC064800C49A765EFC17A7|yrkI]

  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-C463 (yrkH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2461 NBRP B. subtilis, Japan]
• BKE26510 ([gene|06CC915A18E5ADD42B784B7BBBFD4C344559A34E|yrkH]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE26510 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATACCTCCTATATCAAAT,  downstream forward: _UP4_TAACACAAGTTTCTGCTAAA
• BKK26510 ([gene|06CC915A18E5ADD42B784B7BBBFD4C344559A34E|yrkH]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK26510 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATACCTCCTATATCAAAT,  downstream forward: _UP4_TAACACAAGTTTCTGCTAAA