yrkF

168

putative sulfur carrier protein

locus: BSU_26530

Molecular weight: 20.51 kDa

pI: 5.99

Protein length: 185 aa

Gene length: 558 bp

function: unknown

product: unknown

essential: no

synonyms: yrkF

List of homologs in different organisms, belongs to COG0607 (Galperin et al., 2021)

Gene

Coordinates: 2,712,577  2,713,134

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The protein

Protein family

• sulfur carrier protein TusA family (with [protein|D996F581E21395C3F9CC064800C49A765EFC17A7|yrkI], according to UniProt)

[wiki|Domains]

• [wiki|Rhodanese domain] (aa 101-185) (according to UniProt)

Structure

• [PDB|1DCJ] (E. coli TusA, corresponds to the N-terminal domain of [protein|5FC3DF596816AA4E8541B324EC6DE6D89C90C8E2|yrkF], aa 8 ... 77, 37% identity)
• [PDB|3R2U] (metallo--lactamase from Staphylococcus aureus, corresponds to the C-terminal domain of [protein|5FC3DF596816AA4E8541B324EC6DE6D89C90C8E2|yrkF], aa 106 ... 179, 38% identity)
• [AF|P54433]

Expression and Regulation

Operons

• genes: [gene|6AD1DD074B2F044C6A4E4B98FABC4E7FD67B3139|yrzM]-[gene|8A3F1BFDF85DD96B7FB4EBC807F71639AF69DE98|yrkD]-[gene|1F1FE531622CD218EE8990CDAC6D2D2F9E86FE3F|yrkE]-[gene|5FC3DF596816AA4E8541B324EC6DE6D89C90C8E2|yrkF]-[gene|06CC915A18E5ADD42B784B7BBBFD4C344559A34E|yrkH]-[gene|D996F581E21395C3F9CC064800C49A765EFC17A7|yrkI]-[gene|F3E842844B315FE438F66995CDFB8BA1C32BDF23|yrkJ]-[gene|1BEBD09273240B6B50DABCB160FCCBC205BB2AAE|yrkK]-[gene|2EA9792C1C7CD89E43742F57FFB7300725B6AEF4|yrkL]

  description: [Pubmed|22383849]

• genes: [gene|6AD1DD074B2F044C6A4E4B98FABC4E7FD67B3139|yrzM]-[gene|8A3F1BFDF85DD96B7FB4EBC807F71639AF69DE98|yrkD]-[gene|1F1FE531622CD218EE8990CDAC6D2D2F9E86FE3F|yrkE]-[gene|5FC3DF596816AA4E8541B324EC6DE6D89C90C8E2|yrkF]-[gene|06CC915A18E5ADD42B784B7BBBFD4C344559A34E|yrkH]-[gene|D996F581E21395C3F9CC064800C49A765EFC17A7|yrkI]

  description: [Pubmed|22383849]

Biological materials

Mutant

• BKE26530 ([gene|5FC3DF596816AA4E8541B324EC6DE6D89C90C8E2|yrkF]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE26530 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTTTTCCTCCTTTTCTT,  downstream forward: _UP4_TAAATAAAAAGCATGATGAA
• BKK26530 ([gene|5FC3DF596816AA4E8541B324EC6DE6D89C90C8E2|yrkF]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK26530 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTTTTCCTCCTTTTCTT,  downstream forward: _UP4_TAAATAAAAAGCATGATGAA