yqkA

168

unknown

locus: BSU_23670

Molecular weight: 38.88 kDa

pI: 8.16

Protein length: 343 aa

Gene length: 1032 bp

function: unknown

product: unknown

essential: no

synonyms: yqkA

List of homologs in different organisms, belongs to COG2320 (Galperin et al., 2021)

Gene

Coordinates: 2,462,193  2,463,224

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The protein

Protein family

• C-terminal part: UPF0157 (GrpB) family (single member, according to UniProt)

[wiki|Domains]

• N-terminal [wiki|N-acetyltransferase domain] (according to UniProt)
• C-terminal GrpB superfamily domain

Structure

• [PDB|2NRK] (GrpB from Enterococcus faecalis, corresponds to the C-terminal domain, aa 153-318)
• [AF|P54564]

Expression and Regulation

Operons

• genes: [gene|5B8A6D282E34683726BD6C36E09A6C36E8F99795|yqjY]-[gene|9F88AC3A792F4EE8AA853849BBCF5065717B1D8B|yqjZ]-[gene|3ECD25A4ED9556E74112285B11FC180A7091D730|yqkA]-[gene|DC1C1D4BE1A7F4E28792A2B8273AFE2C7F90E58C|yqkB]-[gene|30363923724EBD39C27967B25B486C71453190D1|yqkC]

  description: [Pubmed|22383849]

• genes: [gene|F0BC150F59730E64BA9AB3F979544EF6B5CC4E9E|polY2]-[gene|9BC3DF46C944A0FD5CAAB4513C4AF00EFC5FC8D5|yqjX]-[gene|5B8A6D282E34683726BD6C36E09A6C36E8F99795|yqjY]-[gene|9F88AC3A792F4EE8AA853849BBCF5065717B1D8B|yqjZ]-[gene|3ECD25A4ED9556E74112285B11FC180A7091D730|yqkA]-[gene|DC1C1D4BE1A7F4E28792A2B8273AFE2C7F90E58C|yqkB]-[gene|30363923724EBD39C27967B25B486C71453190D1|yqkC]

  description: [Pubmed|22383849]

  regulation

  • induced upon DNA damage ([protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA]) [Pubmed|15469515,16267290]

  regulatory mechanism

  • [protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA]: repression, [Pubmed|15469515,16267290], in [regulon|protein:D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA regulon]

Biological materials

Mutant

• MGNA-C407 (yqkA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2405 NBRP B. subtilis, Japan]
• BKE23670 ([gene|3ECD25A4ED9556E74112285B11FC180A7091D730|yqkA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE23670 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_ATTCAATCATTCGTGTTCTC,  downstream forward: _UP4_TTATGAACATACATGTGACA
• BKK23670 ([gene|3ECD25A4ED9556E74112285B11FC180A7091D730|yqkA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK23670 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_ATTCAATCATTCGTGTTCTC,  downstream forward: _UP4_TTATGAACATACATGTGACA