yqhL

168

membrane protein

locus: BSU_24540

Molecular weight: 14.45 kDa

pI: 10.37

Protein length: 126 aa

Gene length: 381 bp

function: unknown

product: unknown

essential: no

synonyms: yqhL

List of homologs in different organisms, belongs to COG0607 (Galperin et al., 2021)

Gene

Coordinates: 2,544,995 → 2,545,375

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The protein

[wiki|Domains]

• C-terminal [wiki|Rhodanese domain] (aa 39-123) (according to UniProt)

Structure

• [PDB|3ICR] (from B. anthracis, the rhodanese domain, corresponds to aa 45 ... 119 of YqhL) [pubmed|19725515]
• [AF|P54510]

[wiki|Localization]

• membrane associated [Pubmed|18763711]

Expression and Regulation

Operons

• genes: [gene|B818CD783EDCA86E2E9E0504C02A1B74E3604AFC|yqhL]

  description: [Pubmed|29794222]

  regulation

  • the leader mRNA is processed by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|ricA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|ricF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|ricT] complex [Pubmed|29794222]

Biological materials

Mutant

• MGNA-C425 (yqhL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2423 NBRP B. subtilis, Japan]
• BKE24540 (Δ[gene|B818CD783EDCA86E2E9E0504C02A1B74E3604AFC|yqhL]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE24540 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATGTGATTCTACTCCTTT,  downstream forward: _UP4_TAAAGCAAACAGCTGTCTGG
• BKK24540 (Δ[gene|B818CD783EDCA86E2E9E0504C02A1B74E3604AFC|yqhL]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK24540 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATGTGATTCTACTCCTTT,  downstream forward: _UP4_TAAAGCAAACAGCTGTCTGG

References

• 18763711,29794222,19725515