yqfG

168

RNase M16, endoribonuclease, required for the maturation of the 3' end of 16S rRNA

locus: BSU_25320

Molecular weight: 17.61 kDa

pI: 4.19

Protein length: 157 aa

Gene length: 474 bp

function: 16S rRNA maturation

product: RNase M16

essential: yes

synonyms: yqfG, ybeY

List of homologs in different organisms, belongs to COG0319 (Galperin et al., 2021)

Gene

Coordinates: 2,611,808 → 2,612,281

Phenotypes of a mutant

• essential [pubmed|29873764]
• the deletion can be suppressed by an additional deletion of [gene|0472A4346ADA1DC41C9D00E8F3222A0EBB76DFA8|rnr] [pubmed|29873764]

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The protein

Catalyzed reaction/ biological activity

• maturation of the 3' end of 16S rRNA [pubmed|29873764]

Protein family

• Endoribonuclease YbeY family (single member, according to UniProt)

Structure

• [PDB|1TVI] orthologue from ''T. maritima'' (38% identity) [Pubmed|15965736]
• [AF|P46347]

[wiki|Localization]

• cytoplasm (according to UniProt)

Biological materials

Mutant

• BKK25320 (Δ[gene|E0437C86E5F3707FF1DA13C8B98F73130DED8FB4|yqfG]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK25320 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AACGATATCAATCAGTAAAC,  downstream forward: _UP4_AAGCAAAAGGAATTGCTGGA

References

Reviews

• 31464530,35787606

Original Publications

• 20807199,15965736,21325267,23273979,27834201,29873764