ypsC

168

similar to SAM-dependent 23S rRNA methyltransferase

locus: BSU_22170

Molecular weight: 43.35 kDa

pI: 8.01

Protein length: 385 aa

Gene length: 1158 bp

function: rRNA modification

product: 23S rRNA methyltransferase

essential: no

synonyms: ypsC

List of homologs in different organisms, belongs to COG0116 (Galperin et al., 2021)

Gene

Coordinates: 2,330,075  2,331,232

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The protein

Protein family

• [wiki|Methyltransferase superfamily] (according to UniProt)

[wiki|Domains]

• THUMP domain (aa 44-156) (according to UniProt)

Structure

• [PDB|3K0B] (from Listeria monocytogenes, 63% identity)
• [AF|P50840]

Expression and Regulation

Operons

• genes: [gene|C22F7704DC9D36D96FE089ADCEA546D7A3FB3739|gpsB]-[gene|57A79AAF00243B5E058FA901D43AA7B55CA33F63|rnpB]-[gene|0DF27BEAF763545258CE7DD56F65CD5BF74DD887|ypsC]

  description: [Pubmed|22383849]

• genes: [gene|57A79AAF00243B5E058FA901D43AA7B55CA33F63|rnpB]-[gene|0DF27BEAF763545258CE7DD56F65CD5BF74DD887|ypsC]

  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-A416 (ypsC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/416 NBRP B. subtilis, Japan]
• BKE22170 ([gene|0DF27BEAF763545258CE7DD56F65CD5BF74DD887|ypsC]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE22170 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCGTATCACCCTGTCTTT,  downstream forward: _UP4_TAAAAAGAAGACGCTCTGCA
• BKK22170 ([gene|0DF27BEAF763545258CE7DD56F65CD5BF74DD887|ypsC]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK22170 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCGTATCACCCTGTCTTT,  downstream forward: _UP4_TAAAAAGAAGACGCTCTGCA

two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Fabian Commichau]'s lab

References

• 17010378,21710567