yosS

168

dUTP diphosphatase

locus: BSU_20020

Molecular weight: 16.01 kDa

pI: 7.09

Protein length: 142 aa

Gene length: 429 bp

function: nucleotide metabolism

product: dUTP diphosphatase

essential: no

ec: null

synonyms: yosS

List of homologs in different organisms, belongs to COG0756 (Galperin et al., 2021)

Gene

Coordinates: 2,159,268 → 2,159,696

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The protein

Catalyzed reaction/ biological activity

• dUTP + H₂O --> diphosphate + dUMP + H⁺ (according to UniProt)

Protein family

• dUTPase family (with [protein|1E32B355838787D34352674595A63355609D3AC4|yncF], according to UniProt)

Structure

• [PDB|2BAZ] [Pubmed|21358047]
• [AF|O34919]

Paralogous protein(s)

• [protein|1E32B355838787D34352674595A63355609D3AC4|yncF]

Biological materials

Mutant

• BKE20020 (Δ[gene|B004744E17773FDEAB28FEAE98BCA3C744E63A8E|yosS]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE20020 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTATGTATTAGCTCCTTT,  downstream forward: _UP4_TAATTGGATTTTGTATTTGA
• BKK20020 (Δ[gene|B004744E17773FDEAB28FEAE98BCA3C744E63A8E|yosS]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK20020 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTATGTATTAGCTCCTTT,  downstream forward: _UP4_TAATTGGATTTTGTATTTGA

References

• 21358047,19342774,22383849,23897460