yodT

168

similar to adenosylmethionine-8-amino-7-oxononanoate aminotransferase

locus: BSU_19740

Molecular weight: 48.25 kDa

pI: 5.96

Protein length: 444 aa

Gene length: 1332 bp

function: unknown

product: unknown

essential: no

synonyms: yodT

List of homologs in different organisms, belongs to COG0161 (Galperin et al., 2021)

Gene

Coordinates: 2,144,356 → 2,145,690

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The protein

Protein family

• [wiki|Class-III pyridoxal-phosphate-dependent aminotransferase family] (according to UniProt)

[wiki|Cofactors]

• PLP (according to UniProt)

Structure

• [PDB|3I4J] (from Deinococcus Radiodurans 39% identity)
• [AF|O34662]

Paralogous protein(s)

• [protein|E5F6DF14A4C01B2C90F70E37908D3697BC1B5FEF|yhxA], (34,9%)

Expression and Regulation

Operons

• genes: [gene|13DC4296015A28975BC7697F134FB6ECD6B11489|yodT]-[gene|D8A050BF150C6E5B910C24C0E023F0829EC95BAD|yodS]-[gene|138832210AC94B17E081F3D0AF9BCFE3BE63E601|yodR]-[gene|161ADC907D4902001C8751F51CFA762828C26110|yodQ]-[gene|39749B5EADC3C056BAF232E0B586C65B989F7F77|yodP]-[gene|21589FEBDE54C66DE2A3DCFC2D7BF3AEEC84B44C|kamA]-[gene|A4A099EE3368891C2AD6C2D1DCBE7B238227417F|yokU]-[gene|0A33869E05CC9B49699555EA19D0905A55CA35B9|yozE]

  description: [Pubmed|22383849,14523133]

  regulation

  • expressed early during sporuation in the mother cell ([protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]) [Pubmed|14523133]

  sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|14523133], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]

additional information

• translation is likely to require [protein|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|efp] due to the presence of several consecutive proline residues [PubMed|23239624,23239623]

Biological materials

Mutant

• MGNA-B508 (yodT::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1507 NBRP B. subtilis, Japan]
• BKE19740 (Δ[gene|13DC4296015A28975BC7697F134FB6ECD6B11489|yodT]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE19740 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGCTCCCACCTCAAAACC,  downstream forward: _UP4_AACTTAAAAAAGGATTGACA
• BKK19740 (Δ[gene|13DC4296015A28975BC7697F134FB6ECD6B11489|yodT]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK19740 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGCTCCCACCTCAAAACC,  downstream forward: _UP4_AACTTAAAAAAGGATTGACA

References

• 14523133