yocH

yocH
168

peptidoglycan hydrolase (N-acetylglucosamidase)

locus
BSU_19210
Molecular weight
30.03 kDa
pI
8.37
Protein length
Gene length
function
cell wall turnover
product
peptidoglycan hydrolase
essential
no
synonyms
yocH

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG3584 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,092,899 → 2,093,762
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The protein
[wiki|Domains]
contains two N-acetylglucosamine-polymer-binding [wiki|LysM domain]s [Pubmed|18430080]
2 [wiki|LysM domain]s (aa 26-69, aa 78-121) (according to UniProt)
Structure
[PDB|4WLK] ([protein|3DAE76660E0CB213155265A3E8DF78A992883F87|yuiC], corresponds to aa 155 ... 286 of [protein|BEBA71A2EF56DAEAF80924856DA68124805BB158|yocH], 34% identity) [pubmed|26163297]
[AF|O34669]
[wiki|Localization]
extracellular (signal peptide) [Pubmed|18957862]
Expression and Regulation
Operons
genes
[gene|BEBA71A2EF56DAEAF80924856DA68124805BB158|yocH]
description
[Pubmed|12950927]
regulation
expressed under conditions that trigger sporulation ([wiki|Spo0A]) [Pubmed|14651647]
regulatory mechanism
[protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]: activation, [Pubmed|14651647], in [regulon|protein:2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A regulon]
[protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression, [Pubmed|15101989], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]
[protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|walR]: activation, [Pubmed|12950927], in [regulon|protein:7F340423A34CE40D1F1AA8D373F7C4B859A6496D|walR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|12950927], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Open in new tab

[gene|BEBA71A2EF56DAEAF80924856DA68124805BB158|yocH]

2024-03-23 20:57:20

ghost

46

7f79235599d294df06fb81bdfb07a428d4f7fe7c

8D89F7E9C841F6CDBAA36EE4882DF18E91716DFC

genes
[gene|E85341621B3835F63F07C506E08B73A92E588B1F|bsrB]-[gene|E6EA8098E37A4A2F7370920AC1A500EE4319AAA2|recQ]-[gene|BEBA71A2EF56DAEAF80924856DA68124805BB158|yocH]
description
[Pubmed|22383849]
Open in new tab

[gene|E85341621B3835F63F07C506E08B73A92E588B1F|bsrB]→[gene|BEBA71A2EF56DAEAF80924856DA68124805BB158|yocH]

2024-03-11 20:30:25

Jstuelk

70

c46135c8ecda918f2cefaaabf9488391edf3d4e8

9354DC65CD7CD9CA6CB2929005BDACFC092A2FE9

Biological materials
Mutant
MGNA-A321 (yocH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/321 NBRP B. subtilis, Japan]
AH023 knock-out mutant (kan), available in [wiki|Kevin Devine]'s and [wiki|Jörg Stülke]'s labs [Pubmed|17581128]
BKE19210 (''[gene|BEBA71A2EF56DAEAF80924856DA68124805BB158|yocH]''::''erm'', available in the BGSC and in [wiki|Jörg Stülke]'s lab) [pubmed|28189581]
BKE19210 (Δ[gene|BEBA71A2EF56DAEAF80924856DA68124805BB158|yocH]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE19210 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAAGTAAATCCTCCCTTG,  downstream forward: _UP4_TAATAATATAGATTGTGATA
BKK19210 (Δ[gene|BEBA71A2EF56DAEAF80924856DA68124805BB158|yocH]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK19210 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAAGTAAATCCTCCCTTG,  downstream forward: _UP4_TAATAATATAGATTGTGATA
Expression vectors
pGP1691, expression of ''yocH''-Strep in ''B. subtilis'', in [wiki|pGP382], available in [wiki|Jörg Stülke]'s lab
pGP1693, expression of ''yocH''-Strep in ''E. coli'', in [wiki|pGP574], available in [wiki|Jörg Stülke]'s lab
labs
[wiki|Kevin Devine]
References
Reviews
18430080
Original Publications
14651647,15101989,18957862,12950927,20059685,20070526,23199363,17581128,31808740,26163297

BEBA71A2EF56DAEAF80924856DA68124805BB158

Page visits: 4540

Time of last update: 2024-03-28 02:12:33

Author of last update: Jstuelk