yngF

168

methylglutaconyl-CoA hydratase

locus: BSU_18220

Molecular weight: 27.72 kDa

pI: 7.67

Protein length: 260 aa

Gene length: 783 bp

function: mother cell metabolism, leucine utilization

product: methylglutaconyl-CoA hydratase

essential: no

ec: 4.2.1.18

synonyms: yngF

List of homologs in different organisms, belongs to COG1024 (Galperin et al., 2021)

Gene

Coordinates: 1,951,228 → 1,952,010

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The protein

Catalyzed reaction/ biological activity

• 3-methylglutaconyl-CoA --→(S)-3-hydroxy-3-methylglutaryl-CoA [Pubmed|19935659]

Protein family

• [wiki|enoyl-CoA hydratase/isomerase family] (according to UniProt)

Structure

• [PDB|3KQF] (from ''B. anthracis'')
• [AF|O34893]

Paralogous protein(s)

• [protein|25B45D6A90AD5152FDA71718B14767EA86ECA15B|yhaR], (34%)

Expression and Regulation

Operons

• genes: [gene|BA28DFBAF88A4994B0541D1FF2187312FDE1FA15|yngJ]-[gene|5F73ACDEAFC7F1EB26D55195100C3283FC1F587C|yngI]-[gene|CAF58B4BAEB03353CF80A2862590888D07B48E2C|yngH]-[gene|1FBF1512C28BCE04E7D51247D94DC88C72FBFF07|yngHB]-[gene|EEEE69A98DFAA3327FBC04F1422E959D8B44AF20|yngG]-[gene|D5CCFDE9312909B7E5B98876794569357791C76B|yngF]-[gene|2192474E13EAF6D2CF97CB957864FC89ED0118E0|yngE]

  description: [Pubmed|12662922]

  regulation

  • expressed during sporulation in the mother cell ([protein|search|SigE]) [Pubmed|15699190,12662922]

  sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|15699190,12662922], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]

Biological materials

Mutant

• BKE18220 (Δ[gene|D5CCFDE9312909B7E5B98876794569357791C76B|yngF]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE18220 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTGTATGCGTCCTCCCTT,  downstream forward: _UP4_TACAAGGGAATATAAAAGGG
• BKK18220 (Δ[gene|D5CCFDE9312909B7E5B98876794569357791C76B|yngF]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK18220 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTGTATGCGTCCTCCCTT,  downstream forward: _UP4_TACAAGGGAATATAAAAGGG

References

• 15699190,12662922,19935659