ynbB

168

putative C-S lyase, required for [protein|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|efp] modification

locus: BSU_17440

Molecular weight: 45.91 kDa

pI: 5.09

Protein length: 421 aa

Gene length: 1266 bp

function: modification of [protein|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|efp]

product: putative C-S lyase

essential: no

synonyms: ynbB

List of homologs in different organisms, belongs to COG4100 (Galperin et al., 2021)

Gene

Coordinates: 1,876,584  1,877,849

Phenotypes of a mutant

• inactivation suppresses the swarming defect of a [gene|248F0805272FED9B38ECBB31E2872BC9EC163CE0|ymfI] mutant [pubmed|29615499]
• defective in [category|SW.4.1.4|Swarming] motility [pubmed|35638827]

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The protein

[wiki|Cofactors]

• PLP (according to UniProt)

Structure

• [PDB|3JZL] (from ''Listeria monocytogenes str. 4b f2365'', 67% identity, 80% similarity) [Pubmed|15770647], [protein|search|3HT4] (from Bacillus Cereus, 99% indentity)
• [AF|P94479]

Expression and Regulation

Operons

• genes: [gene|9F6B78C1932FB56F7F71430DB16BC862A312407B|ynbA]-[gene|14EFA3BF4E5DD5368EF02B0733300CEF7A4E0DC7|ynbB]-[gene|641C4BDD9702804642E1753A9C779E80FABB3919|glnR]-[gene|68F4E792B99F86AE9E8F06D2200E128937331F5D|glnA]

  description: [Pubmed|22383849]

  regulation

  • the mRNA is processed between [gene|641C4BDD9702804642E1753A9C779E80FABB3919|glnR] and [gene|68F4E792B99F86AE9E8F06D2200E128937331F5D|glnA] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|ricA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|ricF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|ricT] complex [Pubmed|29794222]

Biological materials

Mutant

• MGNA-B092 (ynbB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1091 NBRP B. subtilis, Japan]
• BKE17440 ([gene|14EFA3BF4E5DD5368EF02B0733300CEF7A4E0DC7|ynbB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE17440 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AAACATGTCTTTATATTTCC,  downstream forward: _UP4_TAAATTTTTTAAAAATTTCT
• BKK17440 ([gene|14EFA3BF4E5DD5368EF02B0733300CEF7A4E0DC7|ynbB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK17440 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AAACATGTCTTTATATTTCC,  downstream forward: _UP4_TAAATTTTTTAAAAATTTCT

References

Research papers

• 29615499,35638827